Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture

Sharaniza Ab-Rahim, T. Kamarul, Azlina A. Abbas, L. Selvaratnam

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review


Articular cartilage extracellular matrix (ECM) plays a crucial role in regulating chondrocyte functions via cell-matrix interaction, cytoskeletal organization and integrinmediated signaling. The effects of transforming growth factor- β (TGF- β) and β –estradiol on extracellular matrix have remained controversial in chondrocyte studies though it has been proven that cartilage responds to these factors in vivo. In our study, we examined the effect of these factors on modulating glycosaminoglycan secretion. Articular chondrocytes from rabbits were cultured, and the effects of supplementing 10ng/ml of TGF- β, 10nM of β -estradiol, and a combination of both factors were compared. The synthesis of sulphated glycosaminoglycan (GAG) was shown to be enhanced in the TGF- β treated cultures and when TGF-β and β -estradiol were both used. However, β -estradiol does not appear to affect GAG deposition.

Original languageEnglish
Title of host publication3rd Kuala Lumpur International Conference on Biomedical Engineering 2006
EditorsFatimah Ibrahim, Noor Azuan Abu Osman, Juliana Usman, Nahrizul Adib Kadri
Place of PublicationGermany
PublisherSpringer-Verlag London Ltd.
Number of pages4
ISBN (Electronic)9783540680161
Publication statusPublished - 2007
Externally publishedYes
EventKuala Lumpur International Conference on Biomedical Engineering (BIOMED) 2006 - Palace of the Golden Horses, Kuala Lumpur, Malaysia
Duration: 11 Dec 200614 Dec 2006
Conference number: 3rd
https://link.springer.com/book/10.1007%2F978-3-540-68017-8 (Proceedings)

Publication series

NameIFMBE Proceedings
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277


ConferenceKuala Lumpur International Conference on Biomedical Engineering (BIOMED) 2006
Abbreviated titleBIOMED 2006
CityKuala Lumpur
Internet address


  • Chondrocytes
  • Collagen type II
  • Extracellular matrix (ECM)
  • Glycosaminoglycan

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