Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture

Sharaniza Ab-Rahim; T. Kamarul; Azlina A. Abbas; L. Selvaratnam

doi:10.1007/978-3-540-68017-8_172

Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture

Sharaniza Ab-Rahim, T. Kamarul, Azlina A. Abbas, L. Selvaratnam

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

Abstract

Articular cartilage extracellular matrix (ECM) plays a crucial role in regulating chondrocyte functions via cell-matrix interaction, cytoskeletal organization and integrinmediated signaling. The effects of transforming growth factor- β (TGF- β) and β –estradiol on extracellular matrix have remained controversial in chondrocyte studies though it has been proven that cartilage responds to these factors in vivo. In our study, we examined the effect of these factors on modulating glycosaminoglycan secretion. Articular chondrocytes from rabbits were cultured, and the effects of supplementing 10ng/ml of TGF- β, 10nM of β -estradiol, and a combination of both factors were compared. The synthesis of sulphated glycosaminoglycan (GAG) was shown to be enhanced in the TGF- β treated cultures and when TGF-β and β -estradiol were both used. However, β -estradiol does not appear to affect GAG deposition.

Original language	English
Title of host publication	3rd Kuala Lumpur International Conference on Biomedical Engineering 2006
Editors	Fatimah Ibrahim, Noor Azuan Abu Osman, Juliana Usman, Nahrizul Adib Kadri
Place of Publication	Germany
Publisher	Springer
Pages	684-687
Number of pages	4
ISBN (Electronic)	9783540680161
DOIs	https://doi.org/10.1007/978-3-540-68017-8_172
Publication status	Published - 2007
Externally published	Yes
Event	Kuala Lumpur International Conference on Biomedical Engineering (BIOMED) 2006 - Palace of the Golden Horses, Kuala Lumpur, Malaysia Duration: 11 Dec 2006 → 14 Dec 2006 Conference number: 3rd https://link.springer.com/book/10.1007%2F978-3-540-68017-8 (Proceedings) https://link.springer.com/book/10.1007/978-3-540-68017-8

Publication series

Name	IFMBE Proceedings
Volume	15
ISSN (Print)	1680-0737
ISSN (Electronic)	1433-9277

Conference

Conference	Kuala Lumpur International Conference on Biomedical Engineering (BIOMED) 2006
Abbreviated title	BIOMED 2006
Country/Territory	Malaysia
City	Kuala Lumpur
Period	11/12/06 → 14/12/06
Internet address	https://link.springer.com/book/10.1007%2F978-3-540-68017-8 (Proceedings) https://link.springer.com/book/10.1007/978-3-540-68017-8

Keywords

Chondrocytes
Collagen type II
Extracellular matrix (ECM)
Glycosaminoglycan

Access to Document

10.1007/978-3-540-68017-8_172

Cite this

Ab-Rahim, S., Kamarul, T., Abbas, A. A., & Selvaratnam, L. (2007). Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture. In F. Ibrahim, N. A. Abu Osman, J. Usman, & N. A. Kadri (Eds.), 3rd Kuala Lumpur International Conference on Biomedical Engineering 2006 (pp. 684-687). (IFMBE Proceedings; Vol. 15). Springer. https://doi.org/10.1007/978-3-540-68017-8_172

Ab-Rahim, Sharaniza ; Kamarul, T. ; Abbas, Azlina A. et al. / Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture. 3rd Kuala Lumpur International Conference on Biomedical Engineering 2006. editor / Fatimah Ibrahim ; Noor Azuan Abu Osman ; Juliana Usman ; Nahrizul Adib Kadri. Germany : Springer, 2007. pp. 684-687 (IFMBE Proceedings).

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title = "Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture",

abstract = "Articular cartilage extracellular matrix (ECM) plays a crucial role in regulating chondrocyte functions via cell-matrix interaction, cytoskeletal organization and integrinmediated signaling. The effects of transforming growth factor- β (TGF- β) and β –estradiol on extracellular matrix have remained controversial in chondrocyte studies though it has been proven that cartilage responds to these factors in vivo. In our study, we examined the effect of these factors on modulating glycosaminoglycan secretion. Articular chondrocytes from rabbits were cultured, and the effects of supplementing 10ng/ml of TGF- β, 10nM of β -estradiol, and a combination of both factors were compared. The synthesis of sulphated glycosaminoglycan (GAG) was shown to be enhanced in the TGF- β treated cultures and when TGF-β and β -estradiol were both used. However, β -estradiol does not appear to affect GAG deposition.",

keywords = "Chondrocytes, Collagen type II, Extracellular matrix (ECM), Glycosaminoglycan",

author = "Sharaniza Ab-Rahim and T. Kamarul and Abbas, {Azlina A.} and L. Selvaratnam",

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doi = "10.1007/978-3-540-68017-8_172",

language = "English",

series = "IFMBE Proceedings",

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Ab-Rahim, S, Kamarul, T, Abbas, AA & Selvaratnam, L 2007, Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture. in F Ibrahim, NA Abu Osman, J Usman & NA Kadri (eds), 3rd Kuala Lumpur International Conference on Biomedical Engineering 2006. IFMBE Proceedings, vol. 15, Springer, Germany, pp. 684-687, Kuala Lumpur International Conference on Biomedical Engineering (BIOMED) 2006, Kuala Lumpur, Malaysia, 11/12/06. https://doi.org/10.1007/978-3-540-68017-8_172

Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture. / Ab-Rahim, Sharaniza; Kamarul, T.; Abbas, Azlina A. et al.
3rd Kuala Lumpur International Conference on Biomedical Engineering 2006. ed. / Fatimah Ibrahim; Noor Azuan Abu Osman; Juliana Usman; Nahrizul Adib Kadri. Germany: Springer, 2007. p. 684-687 (IFMBE Proceedings; Vol. 15).

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

TY - GEN

T1 - Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture

AU - Ab-Rahim, Sharaniza

AU - Kamarul, T.

AU - Abbas, Azlina A.

AU - Selvaratnam, L.

N1 - Conference code: 3rd

PY - 2007

Y1 - 2007

N2 - Articular cartilage extracellular matrix (ECM) plays a crucial role in regulating chondrocyte functions via cell-matrix interaction, cytoskeletal organization and integrinmediated signaling. The effects of transforming growth factor- β (TGF- β) and β –estradiol on extracellular matrix have remained controversial in chondrocyte studies though it has been proven that cartilage responds to these factors in vivo. In our study, we examined the effect of these factors on modulating glycosaminoglycan secretion. Articular chondrocytes from rabbits were cultured, and the effects of supplementing 10ng/ml of TGF- β, 10nM of β -estradiol, and a combination of both factors were compared. The synthesis of sulphated glycosaminoglycan (GAG) was shown to be enhanced in the TGF- β treated cultures and when TGF-β and β -estradiol were both used. However, β -estradiol does not appear to affect GAG deposition.

AB - Articular cartilage extracellular matrix (ECM) plays a crucial role in regulating chondrocyte functions via cell-matrix interaction, cytoskeletal organization and integrinmediated signaling. The effects of transforming growth factor- β (TGF- β) and β –estradiol on extracellular matrix have remained controversial in chondrocyte studies though it has been proven that cartilage responds to these factors in vivo. In our study, we examined the effect of these factors on modulating glycosaminoglycan secretion. Articular chondrocytes from rabbits were cultured, and the effects of supplementing 10ng/ml of TGF- β, 10nM of β -estradiol, and a combination of both factors were compared. The synthesis of sulphated glycosaminoglycan (GAG) was shown to be enhanced in the TGF- β treated cultures and when TGF-β and β -estradiol were both used. However, β -estradiol does not appear to affect GAG deposition.

KW - Chondrocytes

KW - Collagen type II

KW - Extracellular matrix (ECM)

KW - Glycosaminoglycan

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U2 - 10.1007/978-3-540-68017-8_172

DO - 10.1007/978-3-540-68017-8_172

M3 - Conference Paper

AN - SCOPUS:84928886589

T3 - IFMBE Proceedings

SP - 684

EP - 687

BT - 3rd Kuala Lumpur International Conference on Biomedical Engineering 2006

A2 - Ibrahim, Fatimah

A2 - Abu Osman, Noor Azuan

A2 - Usman, Juliana

A2 - Kadri, Nahrizul Adib

PB - Springer

CY - Germany

T2 - Kuala Lumpur International Conference on Biomedical Engineering (BIOMED) 2006

Y2 - 11 December 2006 through 14 December 2006

ER -

Ab-Rahim S, Kamarul T, Abbas AA, Selvaratnam L. Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture. In Ibrahim F, Abu Osman NA, Usman J, Kadri NA, editors, 3rd Kuala Lumpur International Conference on Biomedical Engineering 2006. Germany: Springer. 2007. p. 684-687. (IFMBE Proceedings). doi: 10.1007/978-3-540-68017-8_172

Effect of TGF-β and β -estradiol on extracellular matrix secretion in articular chondrocyte culture

Abstract

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Keywords

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