Effect of storage duration on cytokine stability in human serum and plasma

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Quantification of analytes such as cytokines in serum samples is intrinsic to translational research in immune diseases. Optimising pre-analytical conditions is critical for ensuring study quality, including evaluation of cytokine stability. We aimed to evaluate the effect on cytokine stability of storage duration prior to freezing of serum, and compare to plasma samples obtained from patients with systemic lupus erythematosus (SLE). Protein stability was analysed by simultaneously quantifying 18 analytes using a custom multi-analyte profile in SLE patient serum and plasma samples that had been prospectively stored at 4 °C for pre-determined periods between 0 and 30 days, prior to freezing. Six analytes were excluded from analysis, because most tested samples were above or below the limit of detection. Amongst the 12 analysed proteins, 11 did not show significant signal degradation. Significant signal degradation was observed from the fourth day of storage for a single analyte, CCL19. Proteins levels were more stable in unseparated serum compared to plasma for most analytes, with the exception of IL-37 which appears slightly more stable in plasma. Based on this, a maximum 3 days of storage at 4 °C for unseparated serum samples is recommended for biobanked samples intended for cytokine analysis in studies of human immune disease.

Original languageEnglish
Pages (from-to)453-457
Number of pages5
JournalCytokine
Volume113
DOIs
Publication statusPublished - Jan 2019

Keywords

  • Biobank
  • CCL19
  • Cytokine
  • Plasma
  • Serum
  • Storage duration

Cite this

@article{bf2d0a5b292c4e74b349fa579324c268,
title = "Effect of storage duration on cytokine stability in human serum and plasma",
abstract = "Quantification of analytes such as cytokines in serum samples is intrinsic to translational research in immune diseases. Optimising pre-analytical conditions is critical for ensuring study quality, including evaluation of cytokine stability. We aimed to evaluate the effect on cytokine stability of storage duration prior to freezing of serum, and compare to plasma samples obtained from patients with systemic lupus erythematosus (SLE). Protein stability was analysed by simultaneously quantifying 18 analytes using a custom multi-analyte profile in SLE patient serum and plasma samples that had been prospectively stored at 4 °C for pre-determined periods between 0 and 30 days, prior to freezing. Six analytes were excluded from analysis, because most tested samples were above or below the limit of detection. Amongst the 12 analysed proteins, 11 did not show significant signal degradation. Significant signal degradation was observed from the fourth day of storage for a single analyte, CCL19. Proteins levels were more stable in unseparated serum compared to plasma for most analytes, with the exception of IL-37 which appears slightly more stable in plasma. Based on this, a maximum 3 days of storage at 4 °C for unseparated serum samples is recommended for biobanked samples intended for cytokine analysis in studies of human immune disease.",
keywords = "Biobank, CCL19, Cytokine, Plasma, Serum, Storage duration",
author = "Vincent, {Fabien Bernard} and Nim, {Hieu T} and Lee, {Jacinta P. W.} and Morand, {Eric Francis} and James Harris",
year = "2019",
month = "1",
doi = "10.1016/j.cyto.2018.06.009",
language = "English",
volume = "113",
pages = "453--457",
journal = "Cytokine",
issn = "1096-0023",
publisher = "Academic Press",

}

Effect of storage duration on cytokine stability in human serum and plasma. / Vincent, Fabien Bernard; Nim, Hieu T; Lee, Jacinta P. W.; Morand, Eric Francis; Harris, James.

In: Cytokine, Vol. 113, 01.2019, p. 453-457.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Effect of storage duration on cytokine stability in human serum and plasma

AU - Vincent, Fabien Bernard

AU - Nim, Hieu T

AU - Lee, Jacinta P. W.

AU - Morand, Eric Francis

AU - Harris, James

PY - 2019/1

Y1 - 2019/1

N2 - Quantification of analytes such as cytokines in serum samples is intrinsic to translational research in immune diseases. Optimising pre-analytical conditions is critical for ensuring study quality, including evaluation of cytokine stability. We aimed to evaluate the effect on cytokine stability of storage duration prior to freezing of serum, and compare to plasma samples obtained from patients with systemic lupus erythematosus (SLE). Protein stability was analysed by simultaneously quantifying 18 analytes using a custom multi-analyte profile in SLE patient serum and plasma samples that had been prospectively stored at 4 °C for pre-determined periods between 0 and 30 days, prior to freezing. Six analytes were excluded from analysis, because most tested samples were above or below the limit of detection. Amongst the 12 analysed proteins, 11 did not show significant signal degradation. Significant signal degradation was observed from the fourth day of storage for a single analyte, CCL19. Proteins levels were more stable in unseparated serum compared to plasma for most analytes, with the exception of IL-37 which appears slightly more stable in plasma. Based on this, a maximum 3 days of storage at 4 °C for unseparated serum samples is recommended for biobanked samples intended for cytokine analysis in studies of human immune disease.

AB - Quantification of analytes such as cytokines in serum samples is intrinsic to translational research in immune diseases. Optimising pre-analytical conditions is critical for ensuring study quality, including evaluation of cytokine stability. We aimed to evaluate the effect on cytokine stability of storage duration prior to freezing of serum, and compare to plasma samples obtained from patients with systemic lupus erythematosus (SLE). Protein stability was analysed by simultaneously quantifying 18 analytes using a custom multi-analyte profile in SLE patient serum and plasma samples that had been prospectively stored at 4 °C for pre-determined periods between 0 and 30 days, prior to freezing. Six analytes were excluded from analysis, because most tested samples were above or below the limit of detection. Amongst the 12 analysed proteins, 11 did not show significant signal degradation. Significant signal degradation was observed from the fourth day of storage for a single analyte, CCL19. Proteins levels were more stable in unseparated serum compared to plasma for most analytes, with the exception of IL-37 which appears slightly more stable in plasma. Based on this, a maximum 3 days of storage at 4 °C for unseparated serum samples is recommended for biobanked samples intended for cytokine analysis in studies of human immune disease.

KW - Biobank

KW - CCL19

KW - Cytokine

KW - Plasma

KW - Serum

KW - Storage duration

UR - http://www.scopus.com/inward/record.url?scp=85048402513&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2018.06.009

DO - 10.1016/j.cyto.2018.06.009

M3 - Article

VL - 113

SP - 453

EP - 457

JO - Cytokine

JF - Cytokine

SN - 1096-0023

ER -