Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Yves SY Hsieh; Deni Taleski; Brendan Wilkinson; Lakshmi C Wijeyewickrema; Ty E Adams; Robert N Pike; Richard J Payne

doi:10.1039/c1cc14773k

Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Yves SY Hsieh, Deni Taleski, Brendan Wilkinson, Lakshmi C Wijeyewickrema, Ty E Adams, Robert N Pike, Richard J Payne

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Other › peer-review

22 Citations (Scopus)

Abstract

Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech Hirudinaria manillensis) is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.

Original language	English
Pages (from-to)	1547 - 1549
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	10
DOIs	https://doi.org/10.1039/c1cc14773k
Publication status	Published - 2012

Access to Document

10.1039/c1cc14773k

Cite this

@article{22b45b0356214ac6ae31c768486f794a,

title = "Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin",

abstract = "Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech Hirudinaria manillensis) is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.",

author = "Hsieh, {Yves SY} and Deni Taleski and Brendan Wilkinson and Wijeyewickrema, {Lakshmi C} and Adams, {Ty E} and Pike, {Robert N} and Payne, {Richard J}",

year = "2012",

doi = "10.1039/c1cc14773k",

language = "English",

volume = "48",

pages = "1547 -- 1549",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "The Royal Society of Chemistry",

number = "10",

}

TY - JOUR

T1 - Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin

AU - Hsieh, Yves SY

AU - Taleski, Deni

AU - Wilkinson, Brendan

AU - Wijeyewickrema, Lakshmi C

AU - Adams, Ty E

AU - Pike, Robert N

AU - Payne, Richard J

PY - 2012

Y1 - 2012

N2 - Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech Hirudinaria manillensis) is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.

AB - Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech Hirudinaria manillensis) is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.

UR - http://pubs.rsc.org/en/content/articlepdf/2012/cc/c1cc14773k

U2 - 10.1039/c1cc14773k

DO - 10.1039/c1cc14773k

M3 - Article

SN - 1359-7345

VL - 48

SP - 1547

EP - 1549

JO - Chemical Communications

JF - Chemical Communications

IS - 10

ER -

Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Abstract

Access to Document

Other files and links

Cite this