Effect of Low Power mm wave Irradiation on sedimentation, coagulation and light absorbance of purified Bovine Serum Albumin

Globular protein Bovine Serum Albumin is water soluble and is sensitive to Low Power mm Wave (Micro wave) irradiation leading to Coagulation at the isoelectric Point.The objective of the work was to study the effect of these radiations on the coagulation and light absorbance of purified bovine serum albumin protein. When a heat stress is induced by a Low Power mm Wave, an aggregation of the molecules leading to coagulation occurs; comparatively at high temperature coefficient. The Bovine Serum Albumin Solution maintained at a pH of 3.5 after irradiation by Low Power mm Wave remained clear, the sedimentation constant was multiples almost 8 times (indicating aggregation and agglutination)when compared to the native sample. When the Bovine Serum Albumin sample was irradiated with a frequency of 72.13 GHz. a small change in sedimentation coefficient took place indicating primary coagulation. When the frequency was increased to 98.52 GHz. there was a rapid increase in the sedimentation coefficient and high level of coagulation indicating almost complete denaturation of Bovine Serum Albumin. As a matter of fact frequency at 114.35 GHz. did not make much change in the sedimentation coefficient and coagulation remained almost constant. Similarly Bovine Serum Albumin treated at 56 °C for 25 minutes showed equivalent coagulation as compared to Low Power mm wave irradiation within the range of 72.13 GHz. to 114. 35 GHz. as analysed by U.V/Vis. Spectroscopy (samples treated with Coomassie Brilliant Blue G250) at 595 nm showed relatively similar absorbance.