Effect of intra-cisternal application of kainic acid on the spinal cord and locomotor activity in rats

Nilesh Kumar Mitra, Tiffanie EW Goh, Thalisha Bala Krishnan, Vishna Devi Nadarajah, Arun Kumar Vasavaraj, Tomoko Soga

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    Abstract

    Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease of idiopathic etiology. Glutamate excitotoxicity is one of the proposed hypotheses causing progressive death of motor neurons. We aimed to develop an experimental animal model of this disease to enhance the knowledge of pathophysiological mechanism of ALS. Male Wistar rats were infused with Kainic acid (KA) intra-cisternally for 5 days at the dosage of 50 fmol/day and 150 fmol/day. Locomotor activity, sensory function and histological changes in cervical and lumbar sections of spinal cord were evaluated. Glial Fibrillary Acidic Protein (GFAP) and Neurofilament Protein (NFP) were used as immunohistochemical marker for reactive astrogliosis and neuronal damage respectively. Specific Superoxide Dismutase (SOD) activity of spinal cord was estimated. The locomotor activity in the parameter of observed mean action time remained reduced on 14th day after administration of KA. Spinal motor neurons under Nissl stain showed pyknosis of nucleus and vacuolation of neuropil. GFAP expression increased significantly in the lumbar section of the spinal cord with high dose of KA treatment (p
    Original languageEnglish
    Pages (from-to)1505 - 1515
    Number of pages11
    JournalInternational Journal of Clinical and Experimental Pathology
    Volume6
    Issue number8
    Publication statusPublished - 2013

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