Effect of granulocyte-macrophage colony-stimulating factor on the generation of epidermal langerhans cells

K. Burnham, Lorraine Robb, C. L. Scott, M. O'Keeffe, Ken Shortman

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22 Citations (Scopus)

Abstract

The role of granulocyte-macrophage colony-stimulating factor (GM-CSF) and Flt3 ligand in the in vivo development of Langerhans cells (LC) was assessed, considering both the steady-state levels of LC in the epidermis and the rate of LC recovery after depletion following lipopolysaccharide (LPS) treatment. The density of LC was determined by counting following IA-specific immunofluorescent staining of epidermal sections from mouse ears. LC levels were compared in beta common chain receptor null (βc-/-) mice that fail to respond to GM-CSF interleukin-5 (IL-5), in GM-CSF transgenic mice with elevated GM-CSF levels, and in mice given daily injections of Flt3 ligand. In the steady state, LC levels were increased in GM-CSF transgenic mice and present at reduced levels in βc-/- mice but unchanged in Flt3 ligand-injected mice. Application of LPS to the ears of control BL/6 mice led to an approximately 76% reduction in LC 4 days later, with recovery beginning by day 8 and a return to normal levels by 2 weeks. This recovery was significantly delayed in βc-/- mice and unchanged in Flt3 ligand-injected mice. These results suggest that GM-CSF (but not Flt3 ligand) enhances recruitment/maturation of LC even though GM-CSF is not essential for their formation.

Original languageEnglish
Pages (from-to)1071-1076
Number of pages6
JournalJournal of Interferon and Cytokine Research
Volume20
Issue number12
DOIs
Publication statusPublished - 2000
Externally publishedYes

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