TY - JOUR
T1 - Effect of extraction temperature on stability of major polyphenols and antioxidant activity of Orthosiphon stamineus leaf
AU - Akowuah, Gabriel A.
AU - Zhari, Ismail
N1 - Funding Information:
Received July 5, 2008. Financial support from the Ministry of Science, Technology, and Environment of Malaysia is gratefully acknowledged. Address correspondence to Gabriel A. Akowuah, School of Pharmacy, UCSI University, 56000 Cheras, Kuala Lumpur, Malaysia. E-mail: [email protected]
PY - 2010/7
Y1 - 2010/7
N2 - The effect of extraction temperature on the stability of polyphenols and free radical scavenging activity of Orthosiphon stamineus leaf extract was investigated. Sinensetin (SEN) and rosmarinic acid (RA) were selected as flavonoid and non-flavonoid polyphenolic markers of the extract, respectively. The levels of the markers were significantly (p < 0.5) higher at the extraction temperature of 40°C. However, decrease in marker concentration was observed from extraction temperature of 60°C and above. The extracts at lower temperature exhibited significant (p < 0.5) free radical-scavenging activity compared to extracts at elevated temperature owing to their degradation.
AB - The effect of extraction temperature on the stability of polyphenols and free radical scavenging activity of Orthosiphon stamineus leaf extract was investigated. Sinensetin (SEN) and rosmarinic acid (RA) were selected as flavonoid and non-flavonoid polyphenolic markers of the extract, respectively. The levels of the markers were significantly (p < 0.5) higher at the extraction temperature of 40°C. However, decrease in marker concentration was observed from extraction temperature of 60°C and above. The extracts at lower temperature exhibited significant (p < 0.5) free radical-scavenging activity compared to extracts at elevated temperature owing to their degradation.
KW - flavonoid
KW - rosmarinic acid
KW - sinensetin
UR - http://www.scopus.com/inward/record.url?scp=78249279371&partnerID=8YFLogxK
U2 - 10.1080/10496475.2010.509652
DO - 10.1080/10496475.2010.509652
M3 - Article
AN - SCOPUS:78249279371
SN - 1049-6475
VL - 16
SP - 160
EP - 166
JO - Journal of Herbs, Spices and Medicinal Plants
JF - Journal of Herbs, Spices and Medicinal Plants
IS - 3-4
ER -