TY - JOUR
T1 - Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle
AU - Benziane, Boubacar
AU - Widegren, Ulrika
AU - Pirkmajer, Sergej
AU - Henriksson, Jan
AU - Stepto, Nigel
AU - Chibalin, Alexander
PY - 2011
Y1 - 2011
N2 - Phospholemman (PLM, FXYD1) is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). We explored the impact of acute and short-term training exercise on PLM physiology in human skeletal muscle. A group of moderately trained males (n=8) performed a one-hour acute bout of exercise by utilizing a one-legged cycling protocol. Muscle biopsies were taken from vastus lateralis at 0 and 63 min (non-exercised leg) and 30 and 60 min (exercised leg). In a group of sedentary males (n=9), we determined the effect of a 10-day intense aerobic cycle training on Na+,K+-ATPase subunit expression, PLM phosphorylation and total PLM expression as well as PLM phosphorylation in response to acute exercise (1 h at 72 VO2peak). Biopsies were taken at rest, immediately following, and 3 h after an acute exercise bout before and at the conclusion of the 10-day training study. PLM phosphorylation was increased both at Ser63 and Ser68 immediately after acute exercise (75 ; p
AB - Phospholemman (PLM, FXYD1) is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). We explored the impact of acute and short-term training exercise on PLM physiology in human skeletal muscle. A group of moderately trained males (n=8) performed a one-hour acute bout of exercise by utilizing a one-legged cycling protocol. Muscle biopsies were taken from vastus lateralis at 0 and 63 min (non-exercised leg) and 30 and 60 min (exercised leg). In a group of sedentary males (n=9), we determined the effect of a 10-day intense aerobic cycle training on Na+,K+-ATPase subunit expression, PLM phosphorylation and total PLM expression as well as PLM phosphorylation in response to acute exercise (1 h at 72 VO2peak). Biopsies were taken at rest, immediately following, and 3 h after an acute exercise bout before and at the conclusion of the 10-day training study. PLM phosphorylation was increased both at Ser63 and Ser68 immediately after acute exercise (75 ; p
UR - http://ajpendo.physiology.org/content/301/3/E456.full.pdf+html
U2 - 10.1152/ajpendo.00533.2010
DO - 10.1152/ajpendo.00533.2010
M3 - Article
SN - 0193-1849
VL - 301
SP - E456 - E466
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 3
ER -