Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle

Boubacar Benziane, Ulrika Widegren, Sergej Pirkmajer, Jan Henriksson, Nigel Stepto, Alexander Chibalin

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31 Citations (Scopus)


Phospholemman (PLM, FXYD1) is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). We explored the impact of acute and short-term training exercise on PLM physiology in human skeletal muscle. A group of moderately trained males (n=8) performed a one-hour acute bout of exercise by utilizing a one-legged cycling protocol. Muscle biopsies were taken from vastus lateralis at 0 and 63 min (non-exercised leg) and 30 and 60 min (exercised leg). In a group of sedentary males (n=9), we determined the effect of a 10-day intense aerobic cycle training on Na+,K+-ATPase subunit expression, PLM phosphorylation and total PLM expression as well as PLM phosphorylation in response to acute exercise (1 h at 72 VO2peak). Biopsies were taken at rest, immediately following, and 3 h after an acute exercise bout before and at the conclusion of the 10-day training study. PLM phosphorylation was increased both at Ser63 and Ser68 immediately after acute exercise (75 ; p
Original languageEnglish
Pages (from-to)E456 - E466
Number of pages11
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number3
Publication statusPublished - 2011

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