Effect of estradiol and progesterone on phosphatidylinositol metabolism in the uterine epithelium of the mouse

Jane E. Bourke, S. Dank, P. A. Wilce, L. Martin

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The effect of estradiol and progesterone on uterine phosphatidylinositol (PtdIns) metabolism was examined in whole uteri and separated uterine luminal epithelium of ovariectomized mice. Incorporation of [3H]myo-inositol in vitro, into inositol-containing phospholipids extracted from whole uteri, increased in mice injected with estradiol, with maximal incorporation at 9-12 h. The breakdown of PtdIns to inositol polyphosphates was also stimulated in whole uteri by estrogen, with an abrupt increase between 6 and 9 h. Comparable increases in both processes occurred in the uterine epithelium after estrogen stimulation and were inhibited by progesterone pretreatment which by itself had little or no effect. These results suggest that PtdIns metabolism is involved in the stimulation of uterine epithelial cell proliferation by estrogens, and its inhibition by progesterone.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalJournal of Steroid Biochemistry and Molecular Biology
Issue number3
Publication statusPublished - 1 Jan 1991
Externally publishedYes

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