Effect of anti-microbial oligomers on HEK293A cell membrane

Roya Lahiji, Yan Li, Gregory Tew, Mark Banaszak Holl

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

Abstract

This paper presents results from a study of the interaction of amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-1, 2 and 3), a synthetic antimicrobial peptides (AMP) analogs, with HEK 293A (human embryonic kidney) cell line using a high throughput whole cell patch clamp technique. Cells are patched in an Ion Flux 16 instrument and electric response of the cells, due to the damage or pore formation, in the cell membrane is monitored. On average, the increase in the electric signal was 25±5 nA for AMO-1, for AMO-2 was 10±2 nA and for AMO-3 was multiples of few nanoAmpere current steps that added up to 25±5 nA. Our results show that the degree of interaction and disruption of the AMOs with HEK 293A cells are different and dependent on the hydrophobic chain length of them.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages159-162
Number of pages4
Volume3
Publication statusPublished - 9 Aug 2013
Externally publishedYes
EventNanotechnology Conference and Trade Show (Nanotech) 2013 - Washington, United States of America
Duration: 12 May 201316 May 2013

Conference

ConferenceNanotechnology Conference and Trade Show (Nanotech) 2013
Abbreviated titleNanotech 2013 / NSTI 2013
CountryUnited States of America
CityWashington
Period12/05/1316/05/13
OtherNanotechnology 2013: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013

Keywords

  • Antimicrobial oligomers
  • Automated patch clamp
  • Cell membrane
  • HEK 293A cells
  • Ionflux 16

Cite this

Lahiji, R., Li, Y., Tew, G., & Holl, M. B. (2013). Effect of anti-microbial oligomers on HEK293A cell membrane. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 (Vol. 3, pp. 159-162)
Lahiji, Roya ; Li, Yan ; Tew, Gregory ; Holl, Mark Banaszak. / Effect of anti-microbial oligomers on HEK293A cell membrane. Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3 2013. pp. 159-162
@inproceedings{a1cd1414f95041d6b0c44e0e806bbd8e,
title = "Effect of anti-microbial oligomers on HEK293A cell membrane",
abstract = "This paper presents results from a study of the interaction of amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-1, 2 and 3), a synthetic antimicrobial peptides (AMP) analogs, with HEK 293A (human embryonic kidney) cell line using a high throughput whole cell patch clamp technique. Cells are patched in an Ion Flux 16 instrument and electric response of the cells, due to the damage or pore formation, in the cell membrane is monitored. On average, the increase in the electric signal was 25±5 nA for AMO-1, for AMO-2 was 10±2 nA and for AMO-3 was multiples of few nanoAmpere current steps that added up to 25±5 nA. Our results show that the degree of interaction and disruption of the AMOs with HEK 293A cells are different and dependent on the hydrophobic chain length of them.",
keywords = "Antimicrobial oligomers, Automated patch clamp, Cell membrane, HEK 293A cells, Ionflux 16",
author = "Roya Lahiji and Yan Li and Gregory Tew and Holl, {Mark Banaszak}",
year = "2013",
month = "8",
day = "9",
language = "English",
isbn = "9781482205862",
volume = "3",
pages = "159--162",
booktitle = "Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013",

}

Lahiji, R, Li, Y, Tew, G & Holl, MB 2013, Effect of anti-microbial oligomers on HEK293A cell membrane. in Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. vol. 3, pp. 159-162, Nanotechnology Conference and Trade Show (Nanotech) 2013, Washington, United States of America, 12/05/13.

Effect of anti-microbial oligomers on HEK293A cell membrane. / Lahiji, Roya; Li, Yan; Tew, Gregory; Holl, Mark Banaszak.

Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3 2013. p. 159-162.

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

TY - GEN

T1 - Effect of anti-microbial oligomers on HEK293A cell membrane

AU - Lahiji, Roya

AU - Li, Yan

AU - Tew, Gregory

AU - Holl, Mark Banaszak

PY - 2013/8/9

Y1 - 2013/8/9

N2 - This paper presents results from a study of the interaction of amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-1, 2 and 3), a synthetic antimicrobial peptides (AMP) analogs, with HEK 293A (human embryonic kidney) cell line using a high throughput whole cell patch clamp technique. Cells are patched in an Ion Flux 16 instrument and electric response of the cells, due to the damage or pore formation, in the cell membrane is monitored. On average, the increase in the electric signal was 25±5 nA for AMO-1, for AMO-2 was 10±2 nA and for AMO-3 was multiples of few nanoAmpere current steps that added up to 25±5 nA. Our results show that the degree of interaction and disruption of the AMOs with HEK 293A cells are different and dependent on the hydrophobic chain length of them.

AB - This paper presents results from a study of the interaction of amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-1, 2 and 3), a synthetic antimicrobial peptides (AMP) analogs, with HEK 293A (human embryonic kidney) cell line using a high throughput whole cell patch clamp technique. Cells are patched in an Ion Flux 16 instrument and electric response of the cells, due to the damage or pore formation, in the cell membrane is monitored. On average, the increase in the electric signal was 25±5 nA for AMO-1, for AMO-2 was 10±2 nA and for AMO-3 was multiples of few nanoAmpere current steps that added up to 25±5 nA. Our results show that the degree of interaction and disruption of the AMOs with HEK 293A cells are different and dependent on the hydrophobic chain length of them.

KW - Antimicrobial oligomers

KW - Automated patch clamp

KW - Cell membrane

KW - HEK 293A cells

KW - Ionflux 16

UR - http://www.scopus.com/inward/record.url?scp=84881092385&partnerID=8YFLogxK

M3 - Conference Paper

SN - 9781482205862

VL - 3

SP - 159

EP - 162

BT - Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013

ER -

Lahiji R, Li Y, Tew G, Holl MB. Effect of anti-microbial oligomers on HEK293A cell membrane. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3. 2013. p. 159-162