Effect of γ-Tocotrienol and α-Tocopherol on Blood Glutathione and Tumor Marker Enzymes During Chemical Hepatocarcinogenesis in the Rat

Asmah Rahmat, Wan Zurinah Wan Ngah, Zanariah Jarien, Rosnani Ismail, Alini Marzuki, Khalid Abdul Kadir, Nor Aripin Shamaan

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The protective effect of two types of vitamin E (α-tocopherol and γ-tocotrienol) in rats treated with diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were studied by determination of plasma alkaline phosphatase (ALP), plasma and liver microsomal y-glutamyl transpeptidase (GGT) activities, and blood glutathione (GSH). Rats treated with DEN/AAF had significantly elevated plasma and microsomal GGT, plasma ALP activities, and blood GSH levels compared with the normal controls (Ρ<0.05). Supplementation with vitamin E of normal controls did not affect the enzyme activities or blood GSH. in rats treated with DEN/AAF, vitamin E supplementation attenuated GGT and ALP activitiesand blood GSH levels. the optimum dose required for highest attenuation of the tumor marker enzyme activities was 34 mg/kg diet for α-tocopherol and 30 mg/kg diet for y-tocotrienol. Higher doses of the vitamin did not show further attenuation in the level of the tumor marker enzyme activities.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalJournal of Clinical Biochemistry and Nutrition
Issue number3
Publication statusPublished - 1993
Externally publishedYes


  • alkaline phosphatase
  • hepatocarcinogenesis
  • α-glutamyl transpeptidase
  • α-tocopherol
  • γ-tocotrienol

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