Edwardsiella tarda induces plasma membrane ruffles on infection of HEp- 2 cells

Alan D. Phillips, Luiz R. Trabulsi, Gordon Dougan, Gad Frankel

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Interaction of two clinical Edwardsiella tarda isolates with HEp-2 cells was investigated. By electron microscopy we observed at I h post infection that E. tarda induced formation of extensive plasma membrane projections resembling membrane ruffles. The ruffles did not coincide with adhering bacteria. Only few invading bacteria were seen. Vacuolated nuclear membrane was occasionally observed. Three hours post infection, E. tarda induced a contact-dependent cell lysis, revealing the host cell cytoskeleton and nucleus. Only one of the E. tarda strains was seen residing within the host cell remains. The results indicate that E. tarda-induced membrane ruffles may involve a distinct mechanism of bacterial pathogenesis.

Original languageEnglish
Pages (from-to)317-323
Number of pages7
JournalFEMS Microbiology Letters
Issue number2
Publication statusPublished - 15 Apr 1998
Externally publishedYes


  • Edwardsiella tarda
  • Membrane ruffle
  • Salmonella

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