Ectodermal Wnt function as a neural crest inducer

Martin I Garcia-Castro, Christophe Marcelle, Marianne Bronner-Fraser

Research output: Contribution to journalArticleResearchpeer-review

403 Citations (Scopus)

Abstract

Neural crest cells, which generate peripheral nervous system and facial skeleton, arise at the neural plate/ectodermal border via an inductive interaction between these tissues. Wnts and bone morphogenetic proteins (BMPs) play roles in neural crest induction in amphibians and zebrafish. Here, we show that, in avians, Wnt6 is localized in ectoderm and in vivo inhibition of Wnt signaling perturbs neural crest formation. Furthermore, Wnts induce neural crest from naive neural plates in vitro in a defined medium without added factors, whereas BMPs require additives. Our data suggest that Wnt molecules are necessary and sufficient to induce neural crest cells in avian embryos.
Original languageEnglish
Pages (from-to)848 - 851
Number of pages4
JournalScience
Volume297
Issue number5582
Publication statusPublished - 2002

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