Ecological analysis of antigen-specific CTL repertoires defines the relationship between naïve and immune T-cell populations

Paul G. Thomas, Andreas Handel, Peter C. Doherty, Nicole L. La Gruta

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39 Citations (Scopus)


Ecology is typically thought ofas the study of interactions organisms have with each other and their environment and is focused on the distribution and abundance of organisms both within and between environments.Onamolecular level, thecapacitytoprobe analogous questions in the field of T-cell immunology is imperative as we acquire substantial datasets both on epitope-specific T-cell populations through high-resolution analyses of T-cell receptor (TCR) use and on global T-cell populations analyzed via high-throughput DNA sequencing. Here, we present the innovative application of existing statistical measures (used typically in the field of ecology), together with unique statistical analyses, to comprehensively assess how the naïve epitope-specific CD8+ cytotoxic T lymphocyte (CTL) repertoire translates to that found following an influenza-virus-specific immune response. Such interrogation of our extensive, cumulated TCR CDR3β sequence datasets, derived from both naïve and immune CD8+ T-cell populations specific for four different influenza-derived epitopes (DbNP366, influenza nucleoprotein amino acid residues 366-374; DbPA224, influenza acid polymerase amino acid residues 224-233; DbPB1-F2 62, influenza polymerase B 1 reading frame 2 amino acid residues 62-70; KbNS2114, and influenza nonstructural protein 2 amino acid residues 114-121), demonstrates that epitope-specific TCRuseinanantiviral immuneresponseisthe consequenceofacomplex interplay between the intrinsic characteristics of the naïve cytotoxic T lymphocyte precursor pool and extrinsic (likely antigen driven) influences, the contribution of which varies in an epitopespecific fashion.

Original languageEnglish
Pages (from-to)1839-1844
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number5
Publication statusPublished - 29 Jan 2013
Externally publishedYes


  • Antiviral immunity
  • Killer T cells

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