Early lineage priming by trisomy of Erg leads to myeloproliferation in a down syndrome model

Ashley Quan Ping Ng, Yifang Hu, Donald Metcalf, Craig D Hyland, Helen Ierino, Belinda Phipson, Di Wu, Tracey M Baldwin, Maria Kauppi, Hiu Kiu, Ladina Di Rago, Douglas James Hilton, Gordon K Smyth, Warren S Alexander

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Abstract

Down syndrome (DS), with trisomy of chromosome 21 (HSA21), is the commonest human aneuploidy. Pre-leukemic myeloproliferative changes in DS foetal livers precede the acquisition of GATA1 mutations, transient myeloproliferative disorder (DS-TMD) and acute megakaryocytic leukemia (DS-AMKL). Trisomy of the Erg gene is required for myeloproliferation in the Ts(1716)65Dn DS mouse model. We demonstrate here that genetic changes specifically attributable to trisomy of Erg lead to lineage priming of primitive and early multipotential progenitor cells in Ts(1716)65Dn mice, excess megakaryocyte-erythroid progenitors, and malignant myeloproliferation. Gene expression changes dependent on trisomy of Erg in Ts(1716)65Dn multilineage progenitor cells were correlated with those associated with trisomy of HSA21 in human DS hematopoietic stem and primitive progenitor cells. These data suggest a role for ERG as a regulator of hematopoietic lineage potential, and that trisomy of ERG in the context of DS foetal liver hemopoiesis drives the pre-leukemic changes that predispose to subsequent DS-TMD and DS-AMKL.
Original languageEnglish
Article numbere1005211
Number of pages19
JournalPLoS Genetics
Volume11
Issue number5
DOIs
Publication statusPublished - 2015

Cite this

Ng, A. Q. P., Hu, Y., Metcalf, D., Hyland, C. D., Ierino, H., Phipson, B., Wu, D., Baldwin, T. M., Kauppi, M., Kiu, H., Di Rago, L., Hilton, D. J., Smyth, G. K., & Alexander, W. S. (2015). Early lineage priming by trisomy of Erg leads to myeloproliferation in a down syndrome model. PLoS Genetics, 11(5), [e1005211]. https://doi.org/10.1371/journal.pgen.1005211