Early-life exposure to Tris(1,3-dichloroisopropyl) phosphate induces dose-dependent suppression of sexual behavior in male rats

Manami Kamishima, Tatsuya Hattori, Go Suzuki, Hidenori Matsukami, Chiaki Komine, Yasuyuki Horii, Gen Watanabe, Takumi Oti, Hirotaka Sakamoto, Tomoko Soga, Ishwar S. Parhar, Yasuhiko Kondo, Hidetaka Takigami, Maiko Kawaguchi

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8 Citations (Scopus)


Exposure to endocrine-disrupting chemicals may adversely affect animals, particularly during development. Tris(1,3-dichloroisopropyl) phosphate (TDCIPP) is an organophosphate with anti-androgen function in vitro that is present in indoor dust at relatively high concentrations. In male rats, androgens are necessary for the development of reproductive organs, as well as the endocrine and central nervous systems. However, we currently do not know the exact effects of TDCIPP exposure through suckling on subsequent reproductive behavior in males. Here, we show that TDCIPP exposure (25–250 mg kg –1 via oral administration over 28 consecutive days post-birth) suppressed male sexual behavior and reduced testes size. These changes were dose-dependent and appeared first in adults rather than in juveniles. These results demonstrate that TDCIPP exposure led to normal body growth and appearance in juveniles, but disrupted the endocrine system and physiology in adults. Therefore, assays should be performed using adult animals to ensure accuracy, and to confirm the influence of chemical substances given during early mammalian life.

Original languageEnglish
Pages (from-to)649-655
Number of pages7
JournalJournal of Applied Toxicology
Issue number5
Publication statusPublished - May 2018


  • Early-life exposure
  • Endocrine-disrupting chemical
  • Male sexual behavior
  • Oral administration
  • Plasma testosterone
  • Residual chemical substrate, TDCIPP

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