Early establishment of diverse T cell receptor profiles for influenza-specific CD8 + CD62L hi memory T cells

Katherine Kedzierska, Vanessa Venturi, Kenneth Field, Miles P. Davenport, Stephen J. Turner, Peter C. Doherty

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Single-cell analysis of endogenous, primary CD8 + T cell responses to the influenza D b NP 366 and D b PA 224 epitopes indicates that prominent clonotypes bearing "public" or "shared" T cell receptors (TCRs) subset early into CD62L hi and CD62L lo populations. The CD62L lo effectors divide more and are rapidly eliminated during the contraction phase, whereas stable CD62L hi memory populations persist in the long-term. Reflecting the high frequency of small CD62L hi clones expressing "private" TCRs, the TCR diversity range per mouse is generally two times higher within the CD62L hi CD8 + D b NP 366 + set (1.6 times higher for CD62L hi CD8 + D b PA 224 + ) from 8 to >180 days after antigen challenge. Memory CD8 + CD62L hi T cell precursors thus segregate from the outset into populations expressing "best-fit" and "suboptimal" TCR characteristics, with this pattern being maintained stably thereafter. Hence, our analysis suggests that early establishment of influenza-specific memory within the CD8 + CD62L hi subset preserves clonal diversity and prevents "overdominance" by a few public, or shared, clones.

Original languageEnglish
Pages (from-to)9184-9189
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number24
DOIs
Publication statusPublished - 13 Jun 2006
Externally publishedYes

Keywords

  • Influenza A virus
  • T cell receptor repertoire

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