E1-Ngn2/Cre is a new line for regional activation of Cre recombinase in the developing CNS

Joachim Berger, Silke Berger, Raffaella Scardigli, Francois Guillemot, Peter Gruss, Anastassia Stoykova

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Abstract

We generated a transgenic mouse line named E1-Ngn2/Cre that expresses Cre recombinase and GFP under the control of the E1 enhancer element of the gene Ngn2 (Scardigli et al.: Neuron 31:203-217, 2001). Cre-recombinase activity and GFP fluorescence are consistent with the reported expression pattern controlled by the E1-Ngn2 enhancer. Recombination was detected in the progenitor domains p1 and p2 in the ventricular zone of the neural tube and in distinct domains of the pretectum, the dorsal and ventral thalamus, the tegmentum of the mesencephalon, and the hindbrain. In the developing cortex, Cre-recombinase activity is confined to a subpopulation of progenitors predominantly in the region of the ventral and lateral pallium. The E1-Ngn2/Cre mouse line thus provides an excellent novel tool for a region-specific conditional mutagenesis in the developing CNS.
Original languageEnglish
Pages (from-to)195 - 199
Number of pages5
Journalgenesis: The Journal of Genetics and Development
Volume40
Issue number4
Publication statusPublished - 2004
Externally publishedYes

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