DYNLT3 is required for chromosome alignment during mouse oocyte meiotic maturation

Xin Huang, Hai Long Wang, Shu-Tao Qi, Zhen Bo Wang, Jing-Shan Tong, Qing Hua Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhong Quan Qi, Qing-Yuan Sun

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13 Citations (Scopus)

Abstract

Dynein light chain, Tctex-type 3 (DYNLT3), is a member of the cytoplasmic dynein DYNLT light chain family and has been reported to have a potential role in chromosome congression in human mitosis. However, its role in mammalian meiosis is unclear. In this study, we examined its localization, expression, and functions in mouse oocyte meiosis. Immunofluorescent staining showed that DYNLT3 was restricted to the germinal vesicle and associated with kinetochores at the germinal vesicle breakdown stage, metaphase I and metaphase II. The expression level of DYNLT3 was similar at all meiotic stages. Depletion of DYNLT3 by antibody injection resulted in chromosome misalignment and decrease of the polar body extrusion rate. We further found that DYNLT3-depleted oocytes displayed kinetochore-microtubule detachments. Chromosome-spread experiments showed that depletion of DYNLT3 inhibited the metaphase-anaphase transition by preventing homologous chromosome segregation in meiosis I. Our data suggest that DYNLT3 is required for chromosome alignment and homologous chromosome segregation during mouse oocyte meiosis.

Original languageEnglish
Pages (from-to)983-989
Number of pages7
JournalReproductive Sciences
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Keywords

  • DYNLT3
  • kinetochore
  • meiosis
  • microtubule
  • mouse oocyte

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