Dynein light chain association sequences can facilitate nuclear protein import

Gregory William Moseley, Daniela Martino Roth, Michelle De Jesus, Denisse Lorena Leyton, Richard Filmer, Colin William Pouton, David Andrew Jans

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Monitoring Editor: Karsten Weis Nuclear localization sequence (NLS)-dependent nuclear protein import is not conventionally held to require interaction with microtubules (MTs) or components of the MT motor, dynein. Here we report for the first time the role of sequences conferring association with dynein light chains (DLCs) in NLS-dependent nuclear accumulation of the rabies virus P-protein. We find that P-protein nuclear accumulation is significantly enhanced by its dynein light chain association sequence (DLC-AS), dependent on MT integrity and association with DLCs, and that P-protein-DLC complexes can associate with MT cytoskeletal structures. We also find that P-protein DLC-AS, as well as analogous sequences from other proteins, acts as an independent module which can confer enhancement of nuclear accumulation to proteins carrying the P-protein NLS, as well as several heterologous NLSs. Photobleaching experiments in live cells demonstrate that the MT-dependent enhancement of NLS-mediated nuclear accumulation by the P-protein DLC-AS involves an increased rate of nuclear import. This is the first report of DLC-AS enhancement of NLS function, identifying a novel mechanism regulating nuclear transport with relevance to viral and cellular protein biology. Importantly, this data indicates that DLC-AS represent versatile modules to enhance nuclear delivery with potential therapeutic application.
Original languageEnglish
Pages (from-to)3204 - 3213
Number of pages10
JournalMolecular Biology of the Cell
Volume18
Issue number8
Publication statusPublished - 2007

Cite this

Moseley, Gregory William ; Martino Roth, Daniela ; De Jesus, Michelle ; Leyton, Denisse Lorena ; Filmer, Richard ; Pouton, Colin William ; Jans, David Andrew. / Dynein light chain association sequences can facilitate nuclear protein import. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 8. pp. 3204 - 3213.
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title = "Dynein light chain association sequences can facilitate nuclear protein import",
abstract = "Monitoring Editor: Karsten Weis Nuclear localization sequence (NLS)-dependent nuclear protein import is not conventionally held to require interaction with microtubules (MTs) or components of the MT motor, dynein. Here we report for the first time the role of sequences conferring association with dynein light chains (DLCs) in NLS-dependent nuclear accumulation of the rabies virus P-protein. We find that P-protein nuclear accumulation is significantly enhanced by its dynein light chain association sequence (DLC-AS), dependent on MT integrity and association with DLCs, and that P-protein-DLC complexes can associate with MT cytoskeletal structures. We also find that P-protein DLC-AS, as well as analogous sequences from other proteins, acts as an independent module which can confer enhancement of nuclear accumulation to proteins carrying the P-protein NLS, as well as several heterologous NLSs. Photobleaching experiments in live cells demonstrate that the MT-dependent enhancement of NLS-mediated nuclear accumulation by the P-protein DLC-AS involves an increased rate of nuclear import. This is the first report of DLC-AS enhancement of NLS function, identifying a novel mechanism regulating nuclear transport with relevance to viral and cellular protein biology. Importantly, this data indicates that DLC-AS represent versatile modules to enhance nuclear delivery with potential therapeutic application.",
author = "Moseley, {Gregory William} and {Martino Roth}, Daniela and {De Jesus}, Michelle and Leyton, {Denisse Lorena} and Richard Filmer and Pouton, {Colin William} and Jans, {David Andrew}",
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Moseley, GW, Martino Roth, D, De Jesus, M, Leyton, DL, Filmer, R, Pouton, CW & Jans, DA 2007, 'Dynein light chain association sequences can facilitate nuclear protein import' Molecular Biology of the Cell, vol. 18, no. 8, pp. 3204 - 3213.

Dynein light chain association sequences can facilitate nuclear protein import. / Moseley, Gregory William; Martino Roth, Daniela; De Jesus, Michelle; Leyton, Denisse Lorena; Filmer, Richard; Pouton, Colin William; Jans, David Andrew.

In: Molecular Biology of the Cell, Vol. 18, No. 8, 2007, p. 3204 - 3213.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Dynein light chain association sequences can facilitate nuclear protein import

AU - Moseley, Gregory William

AU - Martino Roth, Daniela

AU - De Jesus, Michelle

AU - Leyton, Denisse Lorena

AU - Filmer, Richard

AU - Pouton, Colin William

AU - Jans, David Andrew

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