Dye-ligand expanded bed adsorption of G6PDH from highly dense unclarified yeast extract

Yen Mei Chow, Beng Ti Tey, Mohd Nordin Ibrahim, Arbakariya Ariff, Tau Chuan Ling

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

The application of dye-ligand expanded bed chromatography adsorption (EBA) of glucose-6-phosphate dehydrogenase (G6PDH) from unclarified yeast extract was undertaken by using a commercially available expanded bed column (20 mm i.d.) and UpFront adsorbent (ρ = 1.5 g/mL) from UpFront Chromatography. The influence of biomass concentration on the adsorption capacity was explored by employing yeast extracts containing various biomass concentrations (5-30%, w/v). It was demonstrated that the biomass concentration had little effect on G6PDH adsorption performance. Feedstock containing 15% (w/v) biomass gave a relatively high recovery yield (>90%) of G6PDH compared to feedstock containing 30% (w/v) biomass, which gave a recovery of 75% G6PDH. Nevertheless, the enzyme specific activity of 7 U mg-1 with a purification factor of 6 was achieved in the feedstock containing biomass concentration of 30% (w/v). The generic applicability of dye-ligand as an affinity tool in expanded bed chromatography is discussed.

Original languageEnglish
Pages (from-to)444-448
Number of pages5
JournalProcess Biochemistry
Volume42
Issue number3
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

Keywords

  • Dye-ligand
  • Elution yield
  • Expanded bed adsorption chromatography
  • G6PDH
  • Yeast extracts

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