Dual function of Sdh3 in the respiratory Chain and TIM22 protein translocase of the mitochondrial inner membrane

Natalia Gebert, Michael Gebert, Silke Oeljeklaus, Karina von der Malsburg, David A. Stroud, Bogusz Kulawiak, Christophe Wirth, René P. Zahedi, Pavel Dolezal, Sebastian Wiese, Oliver Simon, Agnes Schulze-Specking, Kaye N. Truscott, Albert Sickmann, Peter Rehling, Bernard Guiard, Carola Hunte, Bettina Warscheid, Martin van der Laan, Nikolaus PfannerNils Wiedemann

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85 Citations (Scopus)

Abstract

The mitochondrial inner membrane harbors the complexes of the respiratory chain and translocase complexes for precursor proteins. We have identified a further subunit of the carrier translocase (TIM22 complex) that surprisingly is identical to subunit 3 of respiratory complex II, succinate dehydrogenase (Sdh3). The membrane-integral protein Sdh3 plays specific functions in electron transfer in complex II. We show by genetic and biochemical approaches that Sdh3 also plays specific functions in the TIM22 complex. Sdh3 forms a subcomplex with Tim18 and is involved in biogenesis and assembly of the membrane-integral subunits of the TIM22 complex. We conclude that the assembly of Sdh3 with different partner proteins, Sdh4 and Tim18, recruits it to two different mitochondrial membrane complexes with functions in bioenergetics and protein biogenesis, respectively. 

Original languageEnglish
Pages (from-to)811-818
Number of pages8
JournalMolecular Cell
Volume44
Issue number5
DOIs
Publication statusPublished - 9 Dec 2011
Externally publishedYes

Keywords

  • Electron Transport Electron Transport Complex II/metabolism Mitochondrial Membrane Transport Proteins/*metabolism Mitochondrial Membranes/enzymology/*metabolism Saccharomyces cerevisiae/cytology/enzymology/*metabolism Saccharomyces cerevisiae Proteins/*metabolism Succinate Dehydrogenase/*metabolism

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