Droplet control technologies for microfluidic high throughput screening (μHTS)

Muhsincan Sesen, Tuncay Alan, Adrian Neild

Research output: Contribution to journalReview ArticleResearchpeer-review

Abstract

The transition from micro well plate and robotics based high throughput screening (HTS) to chip based screening has already started. This transition promises reduced droplet volumes thereby decreasing the amount of fluids used in these studies. Moreover, it significantly boosts throughput allowing screening to keep pace with the overwhelming number of molecular targets being discovered. In this review, we analyse state-of-the-art droplet control technologies that exhibit potential to be used in this new generation of screening devices. Since these systems are enclosed and usually planar, even some of the straightforward methods used in traditional HTS such as pipetting and reading can prove challenging to replicate in microfluidic high throughput screening (μHTS). We critically review the technologies developed for this purpose in depth, describing the underlying physics and discussing the future outlooks.
Original languageEnglish
Pages (from-to)2373-2394
Number of pages23
JournalLab on a Chip
Volume17
Issue number14
DOIs
Publication statusPublished - 2017

Cite this

@article{e5e7453c6903437c8f4bc6b01e9f2270,
title = "Droplet control technologies for microfluidic high throughput screening (μHTS)",
abstract = "The transition from micro well plate and robotics based high throughput screening (HTS) to chip based screening has already started. This transition promises reduced droplet volumes thereby decreasing the amount of fluids used in these studies. Moreover, it significantly boosts throughput allowing screening to keep pace with the overwhelming number of molecular targets being discovered. In this review, we analyse state-of-the-art droplet control technologies that exhibit potential to be used in this new generation of screening devices. Since these systems are enclosed and usually planar, even some of the straightforward methods used in traditional HTS such as pipetting and reading can prove challenging to replicate in microfluidic high throughput screening (μHTS). We critically review the technologies developed for this purpose in depth, describing the underlying physics and discussing the future outlooks.",
author = "Muhsincan Sesen and Tuncay Alan and Adrian Neild",
year = "2017",
doi = "10.1039/c7lc00005g",
language = "English",
volume = "17",
pages = "2373--2394",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "The Royal Society of Chemistry",
number = "14",

}

Droplet control technologies for microfluidic high throughput screening (μHTS). / Sesen, Muhsincan; Alan, Tuncay; Neild, Adrian.

In: Lab on a Chip, Vol. 17, No. 14, 2017, p. 2373-2394.

Research output: Contribution to journalReview ArticleResearchpeer-review

TY - JOUR

T1 - Droplet control technologies for microfluidic high throughput screening (μHTS)

AU - Sesen, Muhsincan

AU - Alan, Tuncay

AU - Neild, Adrian

PY - 2017

Y1 - 2017

N2 - The transition from micro well plate and robotics based high throughput screening (HTS) to chip based screening has already started. This transition promises reduced droplet volumes thereby decreasing the amount of fluids used in these studies. Moreover, it significantly boosts throughput allowing screening to keep pace with the overwhelming number of molecular targets being discovered. In this review, we analyse state-of-the-art droplet control technologies that exhibit potential to be used in this new generation of screening devices. Since these systems are enclosed and usually planar, even some of the straightforward methods used in traditional HTS such as pipetting and reading can prove challenging to replicate in microfluidic high throughput screening (μHTS). We critically review the technologies developed for this purpose in depth, describing the underlying physics and discussing the future outlooks.

AB - The transition from micro well plate and robotics based high throughput screening (HTS) to chip based screening has already started. This transition promises reduced droplet volumes thereby decreasing the amount of fluids used in these studies. Moreover, it significantly boosts throughput allowing screening to keep pace with the overwhelming number of molecular targets being discovered. In this review, we analyse state-of-the-art droplet control technologies that exhibit potential to be used in this new generation of screening devices. Since these systems are enclosed and usually planar, even some of the straightforward methods used in traditional HTS such as pipetting and reading can prove challenging to replicate in microfluidic high throughput screening (μHTS). We critically review the technologies developed for this purpose in depth, describing the underlying physics and discussing the future outlooks.

UR - http://www.scopus.com/inward/record.url?scp=85023177328&partnerID=8YFLogxK

U2 - 10.1039/c7lc00005g

DO - 10.1039/c7lc00005g

M3 - Review Article

VL - 17

SP - 2373

EP - 2394

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 14

ER -