Draft genome sequence of an aminoglycoside-resistant RmtD2-producing Enterobacter cloacae subsp. cloacae ST395 in Brazil

Evelin Rodrigues Martins, Tiago Casella, Maria Fernanda Campagnari Bueno, Gabriela Rodrigues Francisco, Fernanda Modesto Tolentino, Ana Carolina Tegon de Freitas, Louise Cerdeira, Monalisa Nogueira Costa, Carla Cevada, Nilton Lincopan, Doroti de Oliveira Garcia, Mara Corrêa Lelles Nogueira

Research output: Contribution to journalComment / DebateOtherpeer-review

4 Citations (Scopus)


Objectives Enterobacter cloacae has recently emerged as an important agent of nosocomial infections owing to the dissemination of extended-spectrum β-lactamases and carbapenemases in this species. In this context, a rise in the therapeutic use of aminoglycosides was noticed, followed by the accelerated development of resistance mechanisms. In this study, we report the draft genome sequence of a multidrug-resistant E. cloacae subsp. cloacae strain (Ec2) isolated from an active surveillance culture of a patient with Chagas disease. Methods Whole-genome sequencing (WGS) was performed using a Nextera XT DNA library and NextSeq platform. Results WGS analysis revealed the presence of 5527 coding genes, 62 RNA-encoding genes and 275 pseudogenes. Strain Ec2 belongs to sequence type 395 (ST395) and carries 22 transferable antibiotic resistance genes, comprising eight antimicrobial classes, including the rmtD2 gene conferring high-level aminoglycoside resistance. Conclusions This draft genome can be used in comparative genomic analyses with different E. cloacae strains. In addition, it could help at elucidating epidemiological aspects regarding the dissemination of clinically relevant resistance genes.

Original languageEnglish
Pages (from-to)308-309
Number of pages2
JournalJournal of Global Antimicrobial Resistance
Publication statusPublished - Sep 2017
Externally publishedYes


  • Aminoglycoside resistance
  • Enterobacter cloacae
  • rmtD2

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