TY - JOUR
T1 - Draft genome sequence of a multidrug-resistant KPC-2-producing Enterobacter aerogenes isolated from a hospitalised patient in Brazil
AU - Moura, Quézia
AU - Fernandes, Miriam R.
AU - Cerdeira, Louise
AU - Nhambe, Lúcia F.
AU - Ienne, Susan
AU - Souza, Tiago A.
AU - Lincopan, Nilton
PY - 2017/9
Y1 - 2017/9
N2 - Objectives Multidrug-resistant (MDR) Enterobacter aerogenes strains are frequently associated with nosocomial infections and high mortality rates, representing a serious public health problem. The aim of this study was to present the draft genome sequence of a MDR KPC-2-producing E. aerogenes isolated from a perineal swab of a hospitalised patient in Brazil. Methods Genomic DNA was sequenced using an Illumina MiSeq platform. De novo genome assembly was carried out using the A5-Miseq pipeline, and whole-genome sequence analysis was performed using tools from the Center for Genomic Epidemiology. Results The strain harboured resistance genes to β-lactams, aminoglycosides, sulphonamides and trimethoprim in addition to genes encoding multidrug efflux system proteins, a quaternary ammonium transporter and heavy metal efflux system proteins. In addition, the strain harboured genes encoding diverse virulence factors. Conclusion These data might allow a better understanding of the genetic basis of antimicrobial resistance and virulence in E. aerogenes strains.
AB - Objectives Multidrug-resistant (MDR) Enterobacter aerogenes strains are frequently associated with nosocomial infections and high mortality rates, representing a serious public health problem. The aim of this study was to present the draft genome sequence of a MDR KPC-2-producing E. aerogenes isolated from a perineal swab of a hospitalised patient in Brazil. Methods Genomic DNA was sequenced using an Illumina MiSeq platform. De novo genome assembly was carried out using the A5-Miseq pipeline, and whole-genome sequence analysis was performed using tools from the Center for Genomic Epidemiology. Results The strain harboured resistance genes to β-lactams, aminoglycosides, sulphonamides and trimethoprim in addition to genes encoding multidrug efflux system proteins, a quaternary ammonium transporter and heavy metal efflux system proteins. In addition, the strain harboured genes encoding diverse virulence factors. Conclusion These data might allow a better understanding of the genetic basis of antimicrobial resistance and virulence in E. aerogenes strains.
KW - bla
KW - Efflux pumps
KW - Enterobacter aerogenes
KW - Heavy metal resistance
KW - Whole-genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85027411882&partnerID=8YFLogxK
U2 - 10.1016/j.jgar.2017.07.016
DO - 10.1016/j.jgar.2017.07.016
M3 - Comment / Debate
C2 - 28764940
AN - SCOPUS:85027411882
SN - 2213-7165
VL - 10
SP - 277
EP - 278
JO - Journal of Global Antimicrobial Resistance
JF - Journal of Global Antimicrobial Resistance
ER -
