Double-Arm Lanthanide Tags Deliver Narrow Gd3+–Gd3+ Distance Distributions in Double Electron–Electron Resonance (DEER) Measurements

Adarshi P. Welegedara, Yin Yang, Michael D. Lee, James D. Swarbrick, Thomas Huber, Bim Graham, Daniella Goldfarb, Gottfried Otting

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Double-arm cyclen-based Gd3+ tags are shown to produce accurate nanometer scale Gd3+–Gd3+ distance measurements in double electron–electron resonance (DEER) experiments by confining the space accessible to the metal ion. The results show excellent agreement with predictions both for the maximum and width of the measured distance distributions. For distance measurements in proteins, the tags can be attached to two cysteine residues located in positions i and i+4, or i and i+8, of an α-helix. In the latter case, an additional mutation introducing an aspartic acid at position i+4 achieves particularly narrow distribution widths. The concept is demonstrated with cysteine mutants of T4 lysozyme and maltose binding protein. We report the narrowest Gd3+–Gd3+ distance distributions observed to date for a protein. By limiting the contribution of tag mobility to the distances measured, double-arm Gd3+ tags open new opportunities to study the conformational landscape of proteins in solution with high sensitivity.

Original languageEnglish
Pages (from-to)11694-11702
Number of pages9
JournalChemistry - A European Journal
Volume23
Issue number48
DOIs
Publication statusPublished - 25 Aug 2017

Keywords

  • double electron-electron resonance
  • EPR spectroscopy
  • gadolinium
  • protein structures

Cite this

Welegedara, Adarshi P. ; Yang, Yin ; Lee, Michael D. ; Swarbrick, James D. ; Huber, Thomas ; Graham, Bim ; Goldfarb, Daniella ; Otting, Gottfried. / Double-Arm Lanthanide Tags Deliver Narrow Gd3+–Gd3+ Distance Distributions in Double Electron–Electron Resonance (DEER) Measurements. In: Chemistry - A European Journal. 2017 ; Vol. 23, No. 48. pp. 11694-11702.
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title = "Double-Arm Lanthanide Tags Deliver Narrow Gd3+–Gd3+ Distance Distributions in Double Electron–Electron Resonance (DEER) Measurements",
abstract = "Double-arm cyclen-based Gd3+ tags are shown to produce accurate nanometer scale Gd3+–Gd3+ distance measurements in double electron–electron resonance (DEER) experiments by confining the space accessible to the metal ion. The results show excellent agreement with predictions both for the maximum and width of the measured distance distributions. For distance measurements in proteins, the tags can be attached to two cysteine residues located in positions i and i+4, or i and i+8, of an α-helix. In the latter case, an additional mutation introducing an aspartic acid at position i+4 achieves particularly narrow distribution widths. The concept is demonstrated with cysteine mutants of T4 lysozyme and maltose binding protein. We report the narrowest Gd3+–Gd3+ distance distributions observed to date for a protein. By limiting the contribution of tag mobility to the distances measured, double-arm Gd3+ tags open new opportunities to study the conformational landscape of proteins in solution with high sensitivity.",
keywords = "double electron-electron resonance, EPR spectroscopy, gadolinium, protein structures",
author = "Welegedara, {Adarshi P.} and Yin Yang and Lee, {Michael D.} and Swarbrick, {James D.} and Thomas Huber and Bim Graham and Daniella Goldfarb and Gottfried Otting",
year = "2017",
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Welegedara, AP, Yang, Y, Lee, MD, Swarbrick, JD, Huber, T, Graham, B, Goldfarb, D & Otting, G 2017, 'Double-Arm Lanthanide Tags Deliver Narrow Gd3+–Gd3+ Distance Distributions in Double Electron–Electron Resonance (DEER) Measurements' Chemistry - A European Journal, vol. 23, no. 48, pp. 11694-11702. https://doi.org/10.1002/chem.201702521

Double-Arm Lanthanide Tags Deliver Narrow Gd3+–Gd3+ Distance Distributions in Double Electron–Electron Resonance (DEER) Measurements. / Welegedara, Adarshi P.; Yang, Yin; Lee, Michael D.; Swarbrick, James D.; Huber, Thomas; Graham, Bim; Goldfarb, Daniella; Otting, Gottfried.

In: Chemistry - A European Journal, Vol. 23, No. 48, 25.08.2017, p. 11694-11702.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Welegedara, Adarshi P.

AU - Yang, Yin

AU - Lee, Michael D.

AU - Swarbrick, James D.

AU - Huber, Thomas

AU - Graham, Bim

AU - Goldfarb, Daniella

AU - Otting, Gottfried

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