Dopaminergic agents differentially alter β-endorphin processing patterns in the rat pituitary neurointermediate lobe

D. J. Autelitano, A. I. Smith, S. J. Lolait, J. W. Funder

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Abstract

We have established the content and molecular species of immunoreactive β-endorphin (ir-β-END) and immunoreactive N-acetyl-endorphin (ir-Nac-END) in rat neurointermediate lobe by specific radioimmunoassay (RIA) and high-performance liquid chromatography after chronic administration of dopamine (DA) agonists and antagonists. The DA agonist, bromocriptine, reduces tissue levels of all major immunoreactive species, in particular the C-terminally shortened N-acetylated forms. The DA antagonist, haloperidol, proportionally increases all immunoreactive forms, except Nac-β-END1-27, thus altering the relative abundance of this species. These data indicate that DA is involved in the control of both tissue levels and processing of β-END-like peptides in the rat neurointermediate lobe.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalNeuroscience Letters
Volume59
Issue number1
DOIs
Publication statusPublished - 16 Aug 1985
Externally publishedYes

Keywords

  • acetylation
  • dopamine
  • high-performance liquid chromatography
  • neurointermediate lobe
  • processing
  • radioimmunoassay
  • rat
  • β-endorphin

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