Donor-derived Mycoplasma hominis and an apparent cluster of M. hominis cases in solid organ transplant recipients

O C Smibert, H L Wilson, A Sohail, S Narayanasamy, MB Schultz, S A Ballard, J C Kwong, J de Boer, O Morrissey, AY Peleg, Gregory Snell, Miranda Paraskeva, A W J Jenney

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Abstract

Background:
Invasive and disseminated Mycoplasma hominis infections are well recognized, but uncommon complications in solid organ transplant recipients. In a single center, a cluster of M. hominis infections were identified in lung transplant recipients from the same thoracic intensive care unit (ICU). We sought to determine the source(s) of these infections.
Materials/methods:
Medical records of the donor and infected transplant recipients were reviewed for clinical characteristics. Clinical specimens underwent routine processing with subculture on Mycoplasma-specific Hayflick’s agar. M. hominis identification was confirmed using sequencing of the 16S rRNA gene. M. hominis isolates were subjected to whole genome sequencing (WGS) on the Illumina NextSeq platform.
Results:
Three lung transplant recipients presented with invasive M. hominis infections at multiple sites characterized by purulent infections without organisms detected by Gram staining. Each patient had a separate donor; however, pre-transplant bronchoalveolar lavage fluid was only available from the donor for Patient 1, which subsequently grew M. hominis. Phylo- and pan-genomic analyses indicated that the isolates from the donor and the corresponding recipient (Patient 1) were closely related and formed a distinct single clade. In contrast, isolates from Patients 2 and 3 were unrelated and divergent from one another.
Conclusion:
M. hominis should be considered a cause of donor-derived infection. Genomic data suggest donor to recipient transmission of M. hominis. Additional patients co-located in ICU, were found to have genetically unrelated M. hominis isolates, excluding patient to patient transmission.
Original languageEnglish
Pages (from-to)1504-1508
Number of pages5
JournalClinical Infectious Diseases
Volume65
Issue number9
DOIs
Publication statusPublished - 1 Nov 2017

Cite this

Smibert, O C ; Wilson, H L ; Sohail, A ; Narayanasamy, S ; Schultz, MB ; Ballard, S A ; Kwong, J C ; de Boer, J ; Morrissey, O ; Peleg, AY ; Snell, Gregory ; Paraskeva, Miranda ; Jenney, A W J. / Donor-derived Mycoplasma hominis and an apparent cluster of M. hominis cases in solid organ transplant recipients. In: Clinical Infectious Diseases. 2017 ; Vol. 65, No. 9. pp. 1504-1508.
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abstract = "Background:Invasive and disseminated Mycoplasma hominis infections are well recognized, but uncommon complications in solid organ transplant recipients. In a single center, a cluster of M. hominis infections were identified in lung transplant recipients from the same thoracic intensive care unit (ICU). We sought to determine the source(s) of these infections.Materials/methods:Medical records of the donor and infected transplant recipients were reviewed for clinical characteristics. Clinical specimens underwent routine processing with subculture on Mycoplasma-specific Hayflick’s agar. M. hominis identification was confirmed using sequencing of the 16S rRNA gene. M. hominis isolates were subjected to whole genome sequencing (WGS) on the Illumina NextSeq platform.Results:Three lung transplant recipients presented with invasive M. hominis infections at multiple sites characterized by purulent infections without organisms detected by Gram staining. Each patient had a separate donor; however, pre-transplant bronchoalveolar lavage fluid was only available from the donor for Patient 1, which subsequently grew M. hominis. Phylo- and pan-genomic analyses indicated that the isolates from the donor and the corresponding recipient (Patient 1) were closely related and formed a distinct single clade. In contrast, isolates from Patients 2 and 3 were unrelated and divergent from one another.Conclusion:M. hominis should be considered a cause of donor-derived infection. Genomic data suggest donor to recipient transmission of M. hominis. Additional patients co-located in ICU, were found to have genetically unrelated M. hominis isolates, excluding patient to patient transmission.",
author = "Smibert, {O C} and Wilson, {H L} and A Sohail and S Narayanasamy and MB Schultz and Ballard, {S A} and Kwong, {J C} and {de Boer}, J and O Morrissey and AY Peleg and Gregory Snell and Miranda Paraskeva and Jenney, {A W J}",
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Donor-derived Mycoplasma hominis and an apparent cluster of M. hominis cases in solid organ transplant recipients. / Smibert, O C; Wilson, H L; Sohail, A; Narayanasamy, S; Schultz, MB; Ballard, S A; Kwong, J C; de Boer, J; Morrissey, O; Peleg, AY; Snell, Gregory; Paraskeva, Miranda; Jenney, A W J.

In: Clinical Infectious Diseases, Vol. 65, No. 9, 01.11.2017, p. 1504-1508.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Donor-derived Mycoplasma hominis and an apparent cluster of M. hominis cases in solid organ transplant recipients

AU - Smibert, O C

AU - Wilson, H L

AU - Sohail, A

AU - Narayanasamy, S

AU - Schultz, MB

AU - Ballard, S A

AU - Kwong, J C

AU - de Boer, J

AU - Morrissey, O

AU - Peleg, AY

AU - Snell, Gregory

AU - Paraskeva, Miranda

AU - Jenney, A W J

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Background:Invasive and disseminated Mycoplasma hominis infections are well recognized, but uncommon complications in solid organ transplant recipients. In a single center, a cluster of M. hominis infections were identified in lung transplant recipients from the same thoracic intensive care unit (ICU). We sought to determine the source(s) of these infections.Materials/methods:Medical records of the donor and infected transplant recipients were reviewed for clinical characteristics. Clinical specimens underwent routine processing with subculture on Mycoplasma-specific Hayflick’s agar. M. hominis identification was confirmed using sequencing of the 16S rRNA gene. M. hominis isolates were subjected to whole genome sequencing (WGS) on the Illumina NextSeq platform.Results:Three lung transplant recipients presented with invasive M. hominis infections at multiple sites characterized by purulent infections without organisms detected by Gram staining. Each patient had a separate donor; however, pre-transplant bronchoalveolar lavage fluid was only available from the donor for Patient 1, which subsequently grew M. hominis. Phylo- and pan-genomic analyses indicated that the isolates from the donor and the corresponding recipient (Patient 1) were closely related and formed a distinct single clade. In contrast, isolates from Patients 2 and 3 were unrelated and divergent from one another.Conclusion:M. hominis should be considered a cause of donor-derived infection. Genomic data suggest donor to recipient transmission of M. hominis. Additional patients co-located in ICU, were found to have genetically unrelated M. hominis isolates, excluding patient to patient transmission.

AB - Background:Invasive and disseminated Mycoplasma hominis infections are well recognized, but uncommon complications in solid organ transplant recipients. In a single center, a cluster of M. hominis infections were identified in lung transplant recipients from the same thoracic intensive care unit (ICU). We sought to determine the source(s) of these infections.Materials/methods:Medical records of the donor and infected transplant recipients were reviewed for clinical characteristics. Clinical specimens underwent routine processing with subculture on Mycoplasma-specific Hayflick’s agar. M. hominis identification was confirmed using sequencing of the 16S rRNA gene. M. hominis isolates were subjected to whole genome sequencing (WGS) on the Illumina NextSeq platform.Results:Three lung transplant recipients presented with invasive M. hominis infections at multiple sites characterized by purulent infections without organisms detected by Gram staining. Each patient had a separate donor; however, pre-transplant bronchoalveolar lavage fluid was only available from the donor for Patient 1, which subsequently grew M. hominis. Phylo- and pan-genomic analyses indicated that the isolates from the donor and the corresponding recipient (Patient 1) were closely related and formed a distinct single clade. In contrast, isolates from Patients 2 and 3 were unrelated and divergent from one another.Conclusion:M. hominis should be considered a cause of donor-derived infection. Genomic data suggest donor to recipient transmission of M. hominis. Additional patients co-located in ICU, were found to have genetically unrelated M. hominis isolates, excluding patient to patient transmission.

U2 - 10.1093/cid/cix601

DO - 10.1093/cid/cix601

M3 - Article

VL - 65

SP - 1504

EP - 1508

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 9

ER -