TY - JOUR
T1 - Does physical inactivity induce significant changes in human gut microbiota? New answers using the dry immersion hypoactivity model
AU - Jollet, Maxence
AU - Nay, Kevin
AU - Chopard, Angèle
AU - Bareille, Marie Pierre
AU - Beck, Arnaud
AU - Ollendorff, Vincent
AU - Vernus, Barbara
AU - Bonnieu, Anne
AU - Mariadassou, Mahendra
AU - Rué, Olivier
AU - Derbré, Frédéric
AU - Goustard, Bénédicte
AU - Koechlin-Ramonatxo, Christelle
N1 - Funding Information:
Funding: This research was funded by the French Centre National d’Etudes Spatiales (CNES), 4800000997 and 4800001079 grants. The work was also supported by INRAE and Brittany Council for materials and student fellowships.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - Gut microbiota, a major contributor to human health, is influenced by physical activity and diet, and displays a functional cross-talk with skeletal muscle. Conversely, few data are available on the impact of hypoactivity, although sedentary lifestyles are widespread and associated with negative health and socio-economic impacts. The study aim was to determine the effect of Dry Immersion (DI), a severe hypoactivity model, on the human gut microbiota composition. Stool samples were collected from 14 healthy men before and after 5 days of DI to determine the gut microbiota taxonomic profiles by 16S metagenomic sequencing in strictly controlled dietary conditions. The α and β diversities indices were unchanged. However, the operational taxonomic units associated with the Clostridiales order and the Lachnospiraceae family, belonging to the Firmicutes phylum, were significantly increased after DI. Propionate, a short-chain fatty acid metabolized by skeletal muscle, was significantly reduced in post-DI stool samples. The finding that intestine bacteria are sensitive to hypoactivity raises questions about their impact and role in chronic sedentary lifestyles.
AB - Gut microbiota, a major contributor to human health, is influenced by physical activity and diet, and displays a functional cross-talk with skeletal muscle. Conversely, few data are available on the impact of hypoactivity, although sedentary lifestyles are widespread and associated with negative health and socio-economic impacts. The study aim was to determine the effect of Dry Immersion (DI), a severe hypoactivity model, on the human gut microbiota composition. Stool samples were collected from 14 healthy men before and after 5 days of DI to determine the gut microbiota taxonomic profiles by 16S metagenomic sequencing in strictly controlled dietary conditions. The α and β diversities indices were unchanged. However, the operational taxonomic units associated with the Clostridiales order and the Lachnospiraceae family, belonging to the Firmicutes phylum, were significantly increased after DI. Propionate, a short-chain fatty acid metabolized by skeletal muscle, was significantly reduced in post-DI stool samples. The finding that intestine bacteria are sensitive to hypoactivity raises questions about their impact and role in chronic sedentary lifestyles.
KW - Commensal bacteria
KW - Disuse
KW - Flora
KW - Hypoactivity
KW - Micro-gravity
KW - Muscle atrophy
KW - Phyla
KW - Weightlessness
UR - http://www.scopus.com/inward/record.url?scp=85117933928&partnerID=8YFLogxK
U2 - 10.3390/nu13113865
DO - 10.3390/nu13113865
M3 - Article
C2 - 34836120
AN - SCOPUS:85117933928
SN - 2072-6643
VL - 13
JO - Nutrients
JF - Nutrients
IS - 11
M1 - 3865
ER -