Do Beta 2-Glycoprotein i Disulfide Bonds Protect the Human Retina in the Setting of Age-Related Macular Degeneration?

Miao Qi, Mahmoud Abdelatti, Matthew Krilis, Michele C. Madigan, James C Weaver, Robyn H. Guymer, Peter McCluskey, Ying Wang, Saijun Zhou, Steven A Krilis, Bill M Giannakopoulos

Research output: Contribution to journalReview ArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Age-related macular degeneration (AMD) affects the region of the retina that is responsible for high-resolution vision. It is a major cause of blindness in the aging population. This is the first study that examines the association of redox-modified, cysteine-based, post-translational forms of beta 2-glycoprotein I (β2GPI) in the plasma of individuals with early and late stages of patients with AMD compared with controls. Exploration is also undertaken to assess whether the free thiol form of β2GPI versus the oxidized disulfide form have distinct functional properties in the setting of hydrogen peroxide (H2O2)-mediated cell death of an immortalized human retinal pigment epithelium (RPE) cell line. We demonstrate β2GPI in the retina and choroid of patients with AMD. Free thiol β2GPI is shown to protect the immortalized human RPE cell line against H2O2-induced cell death, whereas the oxidized form of β2GPI and free thiol bovine serum albumin were not protective. Free thiol β2GPI levels were significantly decreased in patients with late AMD compared with early AMD and healthy controls. Our observations lead to the hypothesis that free thiol β2GPI may protect against oxidative stress injury to RPE cells in the early stages of AMD. Antioxid. Redox Signal. 24, 32-38.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalAntioxidants and Redox Signaling
Volume24
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Cite this

Qi, Miao ; Abdelatti, Mahmoud ; Krilis, Matthew ; Madigan, Michele C. ; Weaver, James C ; Guymer, Robyn H. ; McCluskey, Peter ; Wang, Ying ; Zhou, Saijun ; Krilis, Steven A ; Giannakopoulos, Bill M. / Do Beta 2-Glycoprotein i Disulfide Bonds Protect the Human Retina in the Setting of Age-Related Macular Degeneration?. In: Antioxidants and Redox Signaling. 2016 ; Vol. 24, No. 1. pp. 32-38.
@article{f51a50e5f6cf4871b471d4561b978c5d,
title = "Do Beta 2-Glycoprotein i Disulfide Bonds Protect the Human Retina in the Setting of Age-Related Macular Degeneration?",
abstract = "Age-related macular degeneration (AMD) affects the region of the retina that is responsible for high-resolution vision. It is a major cause of blindness in the aging population. This is the first study that examines the association of redox-modified, cysteine-based, post-translational forms of beta 2-glycoprotein I (β2GPI) in the plasma of individuals with early and late stages of patients with AMD compared with controls. Exploration is also undertaken to assess whether the free thiol form of β2GPI versus the oxidized disulfide form have distinct functional properties in the setting of hydrogen peroxide (H2O2)-mediated cell death of an immortalized human retinal pigment epithelium (RPE) cell line. We demonstrate β2GPI in the retina and choroid of patients with AMD. Free thiol β2GPI is shown to protect the immortalized human RPE cell line against H2O2-induced cell death, whereas the oxidized form of β2GPI and free thiol bovine serum albumin were not protective. Free thiol β2GPI levels were significantly decreased in patients with late AMD compared with early AMD and healthy controls. Our observations lead to the hypothesis that free thiol β2GPI may protect against oxidative stress injury to RPE cells in the early stages of AMD. Antioxid. Redox Signal. 24, 32-38.",
author = "Miao Qi and Mahmoud Abdelatti and Matthew Krilis and Madigan, {Michele C.} and Weaver, {James C} and Guymer, {Robyn H.} and Peter McCluskey and Ying Wang and Saijun Zhou and Krilis, {Steven A} and Giannakopoulos, {Bill M}",
year = "2016",
month = "1",
day = "1",
doi = "10.1089/ars.2014.6052",
language = "English",
volume = "24",
pages = "32--38",
journal = "Antioxidants and Redox Signaling",
issn = "1523-0864",
publisher = "Mary Ann Liebert Inc",
number = "1",

}

Qi, M, Abdelatti, M, Krilis, M, Madigan, MC, Weaver, JC, Guymer, RH, McCluskey, P, Wang, Y, Zhou, S, Krilis, SA & Giannakopoulos, BM 2016, 'Do Beta 2-Glycoprotein i Disulfide Bonds Protect the Human Retina in the Setting of Age-Related Macular Degeneration?', Antioxidants and Redox Signaling, vol. 24, no. 1, pp. 32-38. https://doi.org/10.1089/ars.2014.6052

Do Beta 2-Glycoprotein i Disulfide Bonds Protect the Human Retina in the Setting of Age-Related Macular Degeneration? / Qi, Miao; Abdelatti, Mahmoud; Krilis, Matthew; Madigan, Michele C.; Weaver, James C; Guymer, Robyn H.; McCluskey, Peter; Wang, Ying; Zhou, Saijun; Krilis, Steven A; Giannakopoulos, Bill M.

In: Antioxidants and Redox Signaling, Vol. 24, No. 1, 01.01.2016, p. 32-38.

Research output: Contribution to journalReview ArticleResearchpeer-review

TY - JOUR

T1 - Do Beta 2-Glycoprotein i Disulfide Bonds Protect the Human Retina in the Setting of Age-Related Macular Degeneration?

AU - Qi, Miao

AU - Abdelatti, Mahmoud

AU - Krilis, Matthew

AU - Madigan, Michele C.

AU - Weaver, James C

AU - Guymer, Robyn H.

AU - McCluskey, Peter

AU - Wang, Ying

AU - Zhou, Saijun

AU - Krilis, Steven A

AU - Giannakopoulos, Bill M

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Age-related macular degeneration (AMD) affects the region of the retina that is responsible for high-resolution vision. It is a major cause of blindness in the aging population. This is the first study that examines the association of redox-modified, cysteine-based, post-translational forms of beta 2-glycoprotein I (β2GPI) in the plasma of individuals with early and late stages of patients with AMD compared with controls. Exploration is also undertaken to assess whether the free thiol form of β2GPI versus the oxidized disulfide form have distinct functional properties in the setting of hydrogen peroxide (H2O2)-mediated cell death of an immortalized human retinal pigment epithelium (RPE) cell line. We demonstrate β2GPI in the retina and choroid of patients with AMD. Free thiol β2GPI is shown to protect the immortalized human RPE cell line against H2O2-induced cell death, whereas the oxidized form of β2GPI and free thiol bovine serum albumin were not protective. Free thiol β2GPI levels were significantly decreased in patients with late AMD compared with early AMD and healthy controls. Our observations lead to the hypothesis that free thiol β2GPI may protect against oxidative stress injury to RPE cells in the early stages of AMD. Antioxid. Redox Signal. 24, 32-38.

AB - Age-related macular degeneration (AMD) affects the region of the retina that is responsible for high-resolution vision. It is a major cause of blindness in the aging population. This is the first study that examines the association of redox-modified, cysteine-based, post-translational forms of beta 2-glycoprotein I (β2GPI) in the plasma of individuals with early and late stages of patients with AMD compared with controls. Exploration is also undertaken to assess whether the free thiol form of β2GPI versus the oxidized disulfide form have distinct functional properties in the setting of hydrogen peroxide (H2O2)-mediated cell death of an immortalized human retinal pigment epithelium (RPE) cell line. We demonstrate β2GPI in the retina and choroid of patients with AMD. Free thiol β2GPI is shown to protect the immortalized human RPE cell line against H2O2-induced cell death, whereas the oxidized form of β2GPI and free thiol bovine serum albumin were not protective. Free thiol β2GPI levels were significantly decreased in patients with late AMD compared with early AMD and healthy controls. Our observations lead to the hypothesis that free thiol β2GPI may protect against oxidative stress injury to RPE cells in the early stages of AMD. Antioxid. Redox Signal. 24, 32-38.

UR - http://www.scopus.com/inward/record.url?scp=84954528302&partnerID=8YFLogxK

U2 - 10.1089/ars.2014.6052

DO - 10.1089/ars.2014.6052

M3 - Review Article

VL - 24

SP - 32

EP - 38

JO - Antioxidants and Redox Signaling

JF - Antioxidants and Redox Signaling

SN - 1523-0864

IS - 1

ER -