DNAM-1 expression marks an alternative program of NK cell maturation

Ludovic Martinet, Lucas Ferrari de Andrade, Camille Guillerey, Jason S Lee, Jing Liu, Fernando Souza-Fonseca-Guimaraes, Dana S Hutchinson, Tatiana B Kolesnik, Sandra E Nicholson, Nicholas D Huntington, Mark J Smyth

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48 Citations (Scopus)

Abstract

Natural killer (NK) cells comprise a heterogeneous population of cells important for pathogen defense and cancer surveillance. However, the functional significance of this diversity is not fully understood. Here, we demonstrate through transcriptional profiling and functional studies that the activating receptor DNAM-1 (CD226) identifies two distinct NK cell functional subsets: DNAM-1(+) and DNAM-1(-) NK cells. DNAM-1(+) NK cells produce high levels of inflammatory cytokines, have enhanced interleukin 15 signaling, and proliferate vigorously. By contrast, DNAM-1(-) NK cells that differentiate from DNAM-1(+) NK cells have greater expression of NK-cell-receptor-related genes and are higher producers of MIP1 chemokines. Collectively, our data reveal the existence of a functional program of NK cell maturation marked by DNAM-1 expression.
Original languageEnglish
Pages (from-to)85 - 97
Number of pages13
JournalCell Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 2015

Cite this

Martinet, L., de Andrade, L. F., Guillerey, C., Lee, J. S., Liu, J., Souza-Fonseca-Guimaraes, F., ... Smyth, M. J. (2015). DNAM-1 expression marks an alternative program of NK cell maturation. Cell Reports, 11(1), 85 - 97. https://doi.org/10.1016/j.celrep.2015.03.006
Martinet, Ludovic ; de Andrade, Lucas Ferrari ; Guillerey, Camille ; Lee, Jason S ; Liu, Jing ; Souza-Fonseca-Guimaraes, Fernando ; Hutchinson, Dana S ; Kolesnik, Tatiana B ; Nicholson, Sandra E ; Huntington, Nicholas D ; Smyth, Mark J. / DNAM-1 expression marks an alternative program of NK cell maturation. In: Cell Reports. 2015 ; Vol. 11, No. 1. pp. 85 - 97.
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abstract = "Natural killer (NK) cells comprise a heterogeneous population of cells important for pathogen defense and cancer surveillance. However, the functional significance of this diversity is not fully understood. Here, we demonstrate through transcriptional profiling and functional studies that the activating receptor DNAM-1 (CD226) identifies two distinct NK cell functional subsets: DNAM-1(+) and DNAM-1(-) NK cells. DNAM-1(+) NK cells produce high levels of inflammatory cytokines, have enhanced interleukin 15 signaling, and proliferate vigorously. By contrast, DNAM-1(-) NK cells that differentiate from DNAM-1(+) NK cells have greater expression of NK-cell-receptor-related genes and are higher producers of MIP1 chemokines. Collectively, our data reveal the existence of a functional program of NK cell maturation marked by DNAM-1 expression.",
author = "Ludovic Martinet and {de Andrade}, {Lucas Ferrari} and Camille Guillerey and Lee, {Jason S} and Jing Liu and Fernando Souza-Fonseca-Guimaraes and Hutchinson, {Dana S} and Kolesnik, {Tatiana B} and Nicholson, {Sandra E} and Huntington, {Nicholas D} and Smyth, {Mark J}",
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Martinet, L, de Andrade, LF, Guillerey, C, Lee, JS, Liu, J, Souza-Fonseca-Guimaraes, F, Hutchinson, DS, Kolesnik, TB, Nicholson, SE, Huntington, ND & Smyth, MJ 2015, 'DNAM-1 expression marks an alternative program of NK cell maturation', Cell Reports, vol. 11, no. 1, pp. 85 - 97. https://doi.org/10.1016/j.celrep.2015.03.006

DNAM-1 expression marks an alternative program of NK cell maturation. / Martinet, Ludovic; de Andrade, Lucas Ferrari; Guillerey, Camille; Lee, Jason S; Liu, Jing; Souza-Fonseca-Guimaraes, Fernando; Hutchinson, Dana S; Kolesnik, Tatiana B; Nicholson, Sandra E; Huntington, Nicholas D; Smyth, Mark J.

In: Cell Reports, Vol. 11, No. 1, 2015, p. 85 - 97.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Martinet, Ludovic

AU - de Andrade, Lucas Ferrari

AU - Guillerey, Camille

AU - Lee, Jason S

AU - Liu, Jing

AU - Souza-Fonseca-Guimaraes, Fernando

AU - Hutchinson, Dana S

AU - Kolesnik, Tatiana B

AU - Nicholson, Sandra E

AU - Huntington, Nicholas D

AU - Smyth, Mark J

PY - 2015

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N2 - Natural killer (NK) cells comprise a heterogeneous population of cells important for pathogen defense and cancer surveillance. However, the functional significance of this diversity is not fully understood. Here, we demonstrate through transcriptional profiling and functional studies that the activating receptor DNAM-1 (CD226) identifies two distinct NK cell functional subsets: DNAM-1(+) and DNAM-1(-) NK cells. DNAM-1(+) NK cells produce high levels of inflammatory cytokines, have enhanced interleukin 15 signaling, and proliferate vigorously. By contrast, DNAM-1(-) NK cells that differentiate from DNAM-1(+) NK cells have greater expression of NK-cell-receptor-related genes and are higher producers of MIP1 chemokines. Collectively, our data reveal the existence of a functional program of NK cell maturation marked by DNAM-1 expression.

AB - Natural killer (NK) cells comprise a heterogeneous population of cells important for pathogen defense and cancer surveillance. However, the functional significance of this diversity is not fully understood. Here, we demonstrate through transcriptional profiling and functional studies that the activating receptor DNAM-1 (CD226) identifies two distinct NK cell functional subsets: DNAM-1(+) and DNAM-1(-) NK cells. DNAM-1(+) NK cells produce high levels of inflammatory cytokines, have enhanced interleukin 15 signaling, and proliferate vigorously. By contrast, DNAM-1(-) NK cells that differentiate from DNAM-1(+) NK cells have greater expression of NK-cell-receptor-related genes and are higher producers of MIP1 chemokines. Collectively, our data reveal the existence of a functional program of NK cell maturation marked by DNAM-1 expression.

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Martinet L, de Andrade LF, Guillerey C, Lee JS, Liu J, Souza-Fonseca-Guimaraes F et al. DNAM-1 expression marks an alternative program of NK cell maturation. Cell Reports. 2015;11(1):85 - 97. https://doi.org/10.1016/j.celrep.2015.03.006