DNA polyplexes formed using PEGylated biodegradable hyperbranched polymers

Lei Tao, William C Chou, Benghoon Tan, Thomas Paul Davis

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26 Citations (Scopus)


A novel PEGylated biodegradable hyperbranched PEG-b-PDMAEMA has been synthesized. The low toxicity, small molecular weight PDMAEMA chains were crosslinked using a biodegradable disulfide-based dimethacrylate (DSDMA) agent to yield higher molecular weight hyperbranched polymers. PEG chains were linked onto the polymer surface, masking the positive charge (as shown by Zeta potential measurements) and reducing the toxicity of the polymer. The hyperbranched structures were also cleaved under reducing conditions and analyzed, confirming the expected component structures. The hyperbranched polymer was mixed with DNA and efficient binding was shown to occur through electrostatic interactions. The hyperbranched structures could be reduced easily, generating lower toxicity oligomer chains. (Chemical Presented).
Original languageEnglish
Pages (from-to)632 - 637
Number of pages6
JournalMacromolecular Bioscience
Issue number6
Publication statusPublished - 2010
Externally publishedYes

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