DNA methylation analysis of candidate genes associated with dementia in peripheral blood

Peter D Fransquet, Paul Lacaze, Richard Saffery, James Phung, Emily Parker, Raj C Shah, Anne M Murray, Robyn Woods, Joanne Ryan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Aim: To investigate whether genes implicated in dementia pathogenesis are differently methylated in peripheral blood. Materials & Methods: Participants included 160 cognitively healthy individuals aged 70+ years: 73 who were subsequently diagnosed with dementia, and 87 controls matched on age, gender, education, smoking and baseline cognition. Forty-nine participants also provided blood samples at diagnosis. Blood DNA methylation of APOE, APP, BDNF, PIN1, SNCA, and TOMM40 was examined. Results: 56 of 299 probes were differentially methylated in dementia compared to controls, and 39 probes prior to diagnosis. The greatest effect size was in APP (cg19423170, Δ-8.32%, Adj.P=0.009 at diagnosis; cg19933173, Δ-4.18%, Adj.P<0.0001 pre-diagnosis). Conclusion: Genes implicated in dementia pathogenesis show differential blood methylation in dementia, even prior to diagnosis.
Original languageEnglish
Number of pages13
JournalEpigenomics
Publication statusAccepted/In press - 22 Sep 2020

Keywords

  • APOE
  • APP
  • BDNF
  • biomarker
  • blood
  • dementia
  • DNA methylation
  • PIN1
  • SNCA
  • TOMM40

Cite this

Fransquet, P. D., Lacaze, P., Saffery, R., Phung, J., Parker, E., Shah, R. C., Murray, A. M., Woods, R., & Ryan, J. (Accepted/In press). DNA methylation analysis of candidate genes associated with dementia in peripheral blood. Epigenomics.