Abstract
Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened tumour cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm.
| Original language | English |
|---|---|
| Pages (from-to) | 207-213 |
| Number of pages | 7 |
| Journal | Cancer Letters |
| Volume | 209 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 25 Jun 2004 |
| Externally published | Yes |
Keywords
- DLC1
- Head and neck squamous cell carcinoma
- Tumour suppressor gene
Cite this
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DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma. / Hewitt, Chelsee; Wilson, Peter; McGlinn, Edwina; MacFarlane, Gary; Papageorgiou, Anne; Woodwards, Robert T.M.; Sloan, Philip; Gollin, Susanne M.; Paterson, Ian; Parkinson, Kenneth K.; Read, Andrew P.; Thakker, Nalin.
In: Cancer Letters, Vol. 209, No. 2, 25.06.2004, p. 207-213.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma
AU - Hewitt, Chelsee
AU - Wilson, Peter
AU - McGlinn, Edwina
AU - MacFarlane, Gary
AU - Papageorgiou, Anne
AU - Woodwards, Robert T.M.
AU - Sloan, Philip
AU - Gollin, Susanne M.
AU - Paterson, Ian
AU - Parkinson, Kenneth K.
AU - Read, Andrew P.
AU - Thakker, Nalin
PY - 2004/6/25
Y1 - 2004/6/25
N2 - Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened tumour cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm.
AB - Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened tumour cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm.
KW - DLC1
KW - Head and neck squamous cell carcinoma
KW - Tumour suppressor gene
UR - http://www.scopus.com/inward/record.url?scp=2442677522&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2003.12.018
DO - 10.1016/j.canlet.2003.12.018
M3 - Article
C2 - 15159023
AN - SCOPUS:2442677522
VL - 209
SP - 207
EP - 213
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -