TY - JOUR
T1 - Diving head-first into brain intravital microscopy
AU - Suthya, Althea R.
AU - Wong, Connie H.Y.
AU - Bourne, Joshua H.
N1 - Funding Information:
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the \u201CContributing to Australian Scholarship Science\u201D (CASS) Foundation (10310 to JB), National Heart Foundation (NHF) Future Leader Fellowship (107214 to CW) and CSL Centenary Fellowship (CW). Funding sources have no role in the research conceptualisation or direction.
Publisher Copyright:
Copyright © 2024 Suthya, Wong and Bourne.
PY - 2024
Y1 - 2024
N2 - Tissue microenvironments during physiology and pathology are highly complex, meaning dynamic cellular activities and their interactions cannot be accurately modelled ex vivo or in vitro. In particular, tissue-specific resident cells which may function and behave differently after isolation and the heterogenous vascular beds in various organs highlight the importance of observing such processes in real-time in vivo. This challenge gave rise to intravital microscopy (IVM), which was discovered over two centuries ago. From the very early techniques of low-optical resolution brightfield microscopy, limited to transparent tissues, IVM techniques have significantly evolved in recent years. Combined with improved animal surgical preparations, modern IVM technologies have achieved significantly higher speed of image acquisition and enhanced image resolution which allow for the visualisation of biological activities within a wider variety of tissue beds. These advancements have dramatically expanded our understanding in cell migration and function, especially in organs which are not easily accessible, such as the brain. In this review, we will discuss the application of rodent IVM in neurobiology in health and disease. In particular, we will outline the capability and limitations of emerging technologies, including photoacoustic, two- and three-photon imaging for brain IVM. In addition, we will discuss the use of these technologies in the context of neuroinflammation.
AB - Tissue microenvironments during physiology and pathology are highly complex, meaning dynamic cellular activities and their interactions cannot be accurately modelled ex vivo or in vitro. In particular, tissue-specific resident cells which may function and behave differently after isolation and the heterogenous vascular beds in various organs highlight the importance of observing such processes in real-time in vivo. This challenge gave rise to intravital microscopy (IVM), which was discovered over two centuries ago. From the very early techniques of low-optical resolution brightfield microscopy, limited to transparent tissues, IVM techniques have significantly evolved in recent years. Combined with improved animal surgical preparations, modern IVM technologies have achieved significantly higher speed of image acquisition and enhanced image resolution which allow for the visualisation of biological activities within a wider variety of tissue beds. These advancements have dramatically expanded our understanding in cell migration and function, especially in organs which are not easily accessible, such as the brain. In this review, we will discuss the application of rodent IVM in neurobiology in health and disease. In particular, we will outline the capability and limitations of emerging technologies, including photoacoustic, two- and three-photon imaging for brain IVM. In addition, we will discuss the use of these technologies in the context of neuroinflammation.
KW - brain
KW - imaging
KW - intravital microscopy
KW - neuroinflammation
KW - stroke
UR - http://www.scopus.com/inward/record.url?scp=85194560649&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2024.1372996
DO - 10.3389/fimmu.2024.1372996
M3 - Review Article
C2 - 38817606
AN - SCOPUS:85194560649
SN - 1664-3224
VL - 15
JO - Frontiers in Immunology
JF - Frontiers in Immunology
M1 - 1372996
ER -