TY - JOUR
T1 - Diverse regulation of cardiac expression of relaxin receptor by alpha1- and Beta1-adrenoceptors
AU - Moore, Xiao-Lei
AU - Su, Yidan
AU - Fan, Yingli
AU - Zhang, You-Yi
AU - Woodcock, Elizabeth A
AU - Dart, Anthony M
AU - Du, Xiao-Jun
PY - 2014
Y1 - 2014
N2 - Relaxin, a new drug for heart failure therapy, exerts its cardiac actions through relaxin family peptide receptor 1 (RXFP1). Factors regulating RXFP1 expression remain unknown. We have investigated effects of activation of adrenoceptors (AR), an important modulator in the development and prognosis of heart failure, on expression of RXFP1 in rat cardiomyocytes and mouse left ventricles (LV). Methods: Expression of RXFP1 at mRNA (real-time PCR) and protein levels (immunoblotting) was measured in cardiomyocytes treated with a- and ?-AR agonists or antagonists. RXFP1 expression was also determined in the LV of transgenic mouse strains with cardiac-restricted overexpression of a1A-, a1B- or ?2-AR. Specific inhibitors were used to explore signal pathways involved in a1-AR mediated regulation of RXFP1 in cardiomyocytes. Results: In cultured cardiomyocytes, a1-AR stimulation resulted in 2-3 fold increase in RXFP1 mRNA (P?
AB - Relaxin, a new drug for heart failure therapy, exerts its cardiac actions through relaxin family peptide receptor 1 (RXFP1). Factors regulating RXFP1 expression remain unknown. We have investigated effects of activation of adrenoceptors (AR), an important modulator in the development and prognosis of heart failure, on expression of RXFP1 in rat cardiomyocytes and mouse left ventricles (LV). Methods: Expression of RXFP1 at mRNA (real-time PCR) and protein levels (immunoblotting) was measured in cardiomyocytes treated with a- and ?-AR agonists or antagonists. RXFP1 expression was also determined in the LV of transgenic mouse strains with cardiac-restricted overexpression of a1A-, a1B- or ?2-AR. Specific inhibitors were used to explore signal pathways involved in a1-AR mediated regulation of RXFP1 in cardiomyocytes. Results: In cultured cardiomyocytes, a1-AR stimulation resulted in 2-3 fold increase in RXFP1 mRNA (P?
UR - http://link.springer.com/article/10.1007%2Fs10557-014-6525-x
UR - https://www.scopus.com/pages/publications/84903769946
U2 - 10.1007/s10557-014-6525-x
DO - 10.1007/s10557-014-6525-x
M3 - Article
SN - 0920-3206
VL - 28
SP - 221
EP - 228
JO - Cardiovascular Drugs and Therapy
JF - Cardiovascular Drugs and Therapy
IS - 3
ER -