Distinctive expression of interleukin-23 receptor subunits on human Th17 and γδ T cells

Bruce D. Wines, May L. Yap, Maree S. Powell, Peck-Szee Tan, Kerry Ko, Eva Orlowski, P. Mark Hogarth

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15 Citations (Scopus)


The interleukin-23 (IL-23) pathway, T helper 17 (Th17) cells and γδ T cells, which respond to IL-23, have major pro-inflammatory roles. We have used unique IL-23 receptor (IL-23R) subunit-specific monoclonal antibodies, X67 and X68, and IL-12 receptor beta-1 subunit (IL-12Rβ1) expression levels to evaluate the IL-23R complex on CD4 β TCR Th17 cells and on γδ T cells. Both IL-23R and IL-12Rβ1 subunits constitute the functional IL-23R. Expression of the IL-23R subunit by cultured Th17 cells was heterogeneous. Th17 cells expressed consistent high levels of the IL-12Rβ1 subunit, which appeared a better predictor of responsiveness to IL-23 than the expression of the IL-23R subunit. Moreover, sorting memory CD4 T cells by high IL-12Rβ1 expression selectively enriched cells committed to IL-17 production from the blood. IL-23R expression was also observed on freshly isolated and cultured γδ T cells and the cultured γδ T cells were not responsive to IL-23.

Original languageEnglish
Pages (from-to)272-279
Number of pages8
JournalImmunology and Cell Biology
Issue number3
Publication statusPublished - 1 Mar 2017

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