Distinct patterns of diversity, population structure and evolution in the AMA1 genes of sympatric Plasmodium falciparum and Plasmodium vivax populations of Papua New Guinea from an area of similarly high transmission

Alicia Arnott, Johanna Aleece Wapling, Ivo Mueller, Paul Allen Ramsland, Peter M Siba, John C Reeder, Alyssa E Barry

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

Background: As Plasmodium falciparum and Plasmodium vivax co-exist in most malaria-endemic regions outside sub-Saharan Africa, malaria control strategies in these areas must target both species in order to succeed. Population genetic analyses can predict the effectiveness of interventions including vaccines, by providing insight into patterns of diversity and evolution. The aim of this study was to investigate the population genetics of leading malaria vaccine candidate AMA1 in sympatric P. falciparum and P. vivax populations of Papua New Guinea (PNG), an area of similarly high prevalence (Pf = 22.3 to 38.8 , Pv = 15.3 to 31.8 ). Methods. A total of 72 Pfama1 and 102 Pvama1 sequences were collected from two distinct areas, Madang and Wosera, on the highly endemic PNG north coast. Results: Despite a greater number of polymorphic sites in the AMA1 genes of P. falciparum (Madang = 52; Wosera = 56) compared to P. vivax (Madang = 36, Wosera = 34), the number of AMA1 haplotypes, haplotype diversity (Hd) and recombination (R) was far lower for P. falciparum (Madang = 12, Wosera = 20; Hd =0.92, R =45.8) than for P. vivax (Madang = 50, Wosera = 38; Hd = 0.99, R = =70.9). Balancing selection was detected only within domain I of AMA1 for P. vivax, and in both domains I and III for P. falciparum. Conclusions: Higher diversity in the genes encoding P. vivax AMA1 than in P. falciparum AMA1 in this highly endemic area has important implications for development of AMA1-based vaccines in PNG and beyond. These results also suggest a smaller effective population size of P. falciparum compared to P. vivax, a finding that warrants further investigation. Differing patterns of selection on the AMA1 genes indicate that critical antigenic sites may differ between the species, highlighting the need for independent investigations of these two leading vaccine candidates. ? 2014 Arnott et al.; licensee BioMed Central Ltd
Original languageEnglish
Pages (from-to)1 - 16
Number of pages16
JournalMalaria Journal
Volume13
Issue number1 (Art. No.:233)
DOIs
Publication statusPublished - 2014

Cite this

@article{05f6664fbca84548b7bfabd025910da9,
title = "Distinct patterns of diversity, population structure and evolution in the AMA1 genes of sympatric Plasmodium falciparum and Plasmodium vivax populations of Papua New Guinea from an area of similarly high transmission",
abstract = "Background: As Plasmodium falciparum and Plasmodium vivax co-exist in most malaria-endemic regions outside sub-Saharan Africa, malaria control strategies in these areas must target both species in order to succeed. Population genetic analyses can predict the effectiveness of interventions including vaccines, by providing insight into patterns of diversity and evolution. The aim of this study was to investigate the population genetics of leading malaria vaccine candidate AMA1 in sympatric P. falciparum and P. vivax populations of Papua New Guinea (PNG), an area of similarly high prevalence (Pf = 22.3 to 38.8 , Pv = 15.3 to 31.8 ). Methods. A total of 72 Pfama1 and 102 Pvama1 sequences were collected from two distinct areas, Madang and Wosera, on the highly endemic PNG north coast. Results: Despite a greater number of polymorphic sites in the AMA1 genes of P. falciparum (Madang = 52; Wosera = 56) compared to P. vivax (Madang = 36, Wosera = 34), the number of AMA1 haplotypes, haplotype diversity (Hd) and recombination (R) was far lower for P. falciparum (Madang = 12, Wosera = 20; Hd =0.92, R =45.8) than for P. vivax (Madang = 50, Wosera = 38; Hd = 0.99, R = =70.9). Balancing selection was detected only within domain I of AMA1 for P. vivax, and in both domains I and III for P. falciparum. Conclusions: Higher diversity in the genes encoding P. vivax AMA1 than in P. falciparum AMA1 in this highly endemic area has important implications for development of AMA1-based vaccines in PNG and beyond. These results also suggest a smaller effective population size of P. falciparum compared to P. vivax, a finding that warrants further investigation. Differing patterns of selection on the AMA1 genes indicate that critical antigenic sites may differ between the species, highlighting the need for independent investigations of these two leading vaccine candidates. ? 2014 Arnott et al.; licensee BioMed Central Ltd",
author = "Alicia Arnott and Wapling, {Johanna Aleece} and Ivo Mueller and Ramsland, {Paul Allen} and Siba, {Peter M} and Reeder, {John C} and Barry, {Alyssa E}",
year = "2014",
doi = "10.1186/1475-2875-13-233",
language = "English",
volume = "13",
pages = "1 -- 16",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "Springer-Verlag London Ltd.",
number = "1 (Art. No.:233)",

}

Distinct patterns of diversity, population structure and evolution in the AMA1 genes of sympatric Plasmodium falciparum and Plasmodium vivax populations of Papua New Guinea from an area of similarly high transmission. / Arnott, Alicia; Wapling, Johanna Aleece; Mueller, Ivo; Ramsland, Paul Allen; Siba, Peter M; Reeder, John C; Barry, Alyssa E.

In: Malaria Journal, Vol. 13, No. 1 (Art. No.:233), 2014, p. 1 - 16.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Distinct patterns of diversity, population structure and evolution in the AMA1 genes of sympatric Plasmodium falciparum and Plasmodium vivax populations of Papua New Guinea from an area of similarly high transmission

AU - Arnott, Alicia

AU - Wapling, Johanna Aleece

AU - Mueller, Ivo

AU - Ramsland, Paul Allen

AU - Siba, Peter M

AU - Reeder, John C

AU - Barry, Alyssa E

PY - 2014

Y1 - 2014

N2 - Background: As Plasmodium falciparum and Plasmodium vivax co-exist in most malaria-endemic regions outside sub-Saharan Africa, malaria control strategies in these areas must target both species in order to succeed. Population genetic analyses can predict the effectiveness of interventions including vaccines, by providing insight into patterns of diversity and evolution. The aim of this study was to investigate the population genetics of leading malaria vaccine candidate AMA1 in sympatric P. falciparum and P. vivax populations of Papua New Guinea (PNG), an area of similarly high prevalence (Pf = 22.3 to 38.8 , Pv = 15.3 to 31.8 ). Methods. A total of 72 Pfama1 and 102 Pvama1 sequences were collected from two distinct areas, Madang and Wosera, on the highly endemic PNG north coast. Results: Despite a greater number of polymorphic sites in the AMA1 genes of P. falciparum (Madang = 52; Wosera = 56) compared to P. vivax (Madang = 36, Wosera = 34), the number of AMA1 haplotypes, haplotype diversity (Hd) and recombination (R) was far lower for P. falciparum (Madang = 12, Wosera = 20; Hd =0.92, R =45.8) than for P. vivax (Madang = 50, Wosera = 38; Hd = 0.99, R = =70.9). Balancing selection was detected only within domain I of AMA1 for P. vivax, and in both domains I and III for P. falciparum. Conclusions: Higher diversity in the genes encoding P. vivax AMA1 than in P. falciparum AMA1 in this highly endemic area has important implications for development of AMA1-based vaccines in PNG and beyond. These results also suggest a smaller effective population size of P. falciparum compared to P. vivax, a finding that warrants further investigation. Differing patterns of selection on the AMA1 genes indicate that critical antigenic sites may differ between the species, highlighting the need for independent investigations of these two leading vaccine candidates. ? 2014 Arnott et al.; licensee BioMed Central Ltd

AB - Background: As Plasmodium falciparum and Plasmodium vivax co-exist in most malaria-endemic regions outside sub-Saharan Africa, malaria control strategies in these areas must target both species in order to succeed. Population genetic analyses can predict the effectiveness of interventions including vaccines, by providing insight into patterns of diversity and evolution. The aim of this study was to investigate the population genetics of leading malaria vaccine candidate AMA1 in sympatric P. falciparum and P. vivax populations of Papua New Guinea (PNG), an area of similarly high prevalence (Pf = 22.3 to 38.8 , Pv = 15.3 to 31.8 ). Methods. A total of 72 Pfama1 and 102 Pvama1 sequences were collected from two distinct areas, Madang and Wosera, on the highly endemic PNG north coast. Results: Despite a greater number of polymorphic sites in the AMA1 genes of P. falciparum (Madang = 52; Wosera = 56) compared to P. vivax (Madang = 36, Wosera = 34), the number of AMA1 haplotypes, haplotype diversity (Hd) and recombination (R) was far lower for P. falciparum (Madang = 12, Wosera = 20; Hd =0.92, R =45.8) than for P. vivax (Madang = 50, Wosera = 38; Hd = 0.99, R = =70.9). Balancing selection was detected only within domain I of AMA1 for P. vivax, and in both domains I and III for P. falciparum. Conclusions: Higher diversity in the genes encoding P. vivax AMA1 than in P. falciparum AMA1 in this highly endemic area has important implications for development of AMA1-based vaccines in PNG and beyond. These results also suggest a smaller effective population size of P. falciparum compared to P. vivax, a finding that warrants further investigation. Differing patterns of selection on the AMA1 genes indicate that critical antigenic sites may differ between the species, highlighting the need for independent investigations of these two leading vaccine candidates. ? 2014 Arnott et al.; licensee BioMed Central Ltd

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085730/pdf/1475-2875-13-233.pdf

U2 - 10.1186/1475-2875-13-233

DO - 10.1186/1475-2875-13-233

M3 - Article

VL - 13

SP - 1

EP - 16

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

IS - 1 (Art. No.:233)

ER -