Dissemination of blaVIM in Greece at the peak of the epidemic of 2005-2006: Clonal expansion of Klebsiella pneumoniae clonal complex 147

C. M. Hasan; A. Turlej-Rogacka; A. C. Vatopoulos; P. Giakkoupi; M. Maatallah; C. G. Giske

doi:10.1111/1469-0691.12187

Dissemination of bla_VIM in Greece at the peak of the epidemic of 2005-2006: Clonal expansion of Klebsiella pneumoniae clonal complex 147

C. M. Hasan, A. Turlej-Rogacka, A. C. Vatopoulos, P. Giakkoupi, M. Maatallah, C. G. Giske

Research output: Contribution to journal › Article › Other › peer-review

34 Citations (Scopus)

Abstract

VIM-producing Klebsiella pneumoniae (n = 21) isolated from ten Greek hospitals during 2003-2007 were characterized with multilocus sequence typing (MLST), semi-automated repetitive sequence-based PCR (rep-PCR) (Diversilab), plasmid replicon typing, serotyping and screening for multiple resistance determinants. The isolates were selected to represent different strain clusters (defined by >80% similarity) according to pulsed-field gel electrophoresis. MLST identified three major clonal complexes (CCs); CC147 (n = 8), CC18 (n = 5) and CC14 (n = 3). Plasmid replicon typing showed that IncA/C and/or IncFII_K replicons were detected among isolates in each of the major CCs. Good concordance was observed between semi-automated-rep PCR genotyping and MLST.

Original language	English
Pages (from-to)	34-37
Number of pages	4
Journal	Clinical Microbiology and Infection
Volume	20
Issue number	1
DOIs	https://doi.org/10.1111/1469-0691.12187
Publication status	Published - Jan 2014
Externally published	Yes

Keywords

MLST
Clonal complex
Metallo-β-lactamase
AmpC
Plasmid replicon

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/1469-0691.12187Licence: CC BY

Cite this

@article{9b5cf171281548178104300e46eac4ce,

title = "Dissemination of blaVIM in Greece at the peak of the epidemic of 2005-2006: Clonal expansion of Klebsiella pneumoniae clonal complex 147",

abstract = "VIM-producing Klebsiella pneumoniae (n = 21) isolated from ten Greek hospitals during 2003-2007 were characterized with multilocus sequence typing (MLST), semi-automated repetitive sequence-based PCR (rep-PCR) (Diversilab), plasmid replicon typing, serotyping and screening for multiple resistance determinants. The isolates were selected to represent different strain clusters (defined by >80\% similarity) according to pulsed-field gel electrophoresis. MLST identified three major clonal complexes (CCs); CC147 (n = 8), CC18 (n = 5) and CC14 (n = 3). Plasmid replicon typing showed that IncA/C and/or IncFIIK replicons were detected among isolates in each of the major CCs. Good concordance was observed between semi-automated-rep PCR genotyping and MLST.",

keywords = "MLST, Clonal complex, Metallo-β-lactamase, AmpC, Plasmid replicon",

author = "Hasan, \{C. M.\} and A. Turlej-Rogacka and Vatopoulos, \{A. C.\} and P. Giakkoupi and M. Maatallah and Giske, \{C. G.\}",

note = "Funding Information: C. G. Giske has received conference support from Calixa Therapeutics Inc and AB Biodisk, and speaker's honoraria from Wyeth, AstraZeneca and Meda. Other authors: none to declare. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2014",

month = jan,

doi = "10.1111/1469-0691.12187",

language = "English",

volume = "20",

pages = "34--37",

journal = "Clinical Microbiology and Infection",

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publisher = "Elsevier",

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}

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T1 - Dissemination of blaVIM in Greece at the peak of the epidemic of 2005-2006

T2 - Clonal expansion of Klebsiella pneumoniae clonal complex 147

AU - Hasan, C. M.

AU - Turlej-Rogacka, A.

AU - Vatopoulos, A. C.

AU - Giakkoupi, P.

AU - Maatallah, M.

AU - Giske, C. G.

N1 - Funding Information: C. G. Giske has received conference support from Calixa Therapeutics Inc and AB Biodisk, and speaker's honoraria from Wyeth, AstraZeneca and Meda. Other authors: none to declare. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2014/1

Y1 - 2014/1

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AB - VIM-producing Klebsiella pneumoniae (n = 21) isolated from ten Greek hospitals during 2003-2007 were characterized with multilocus sequence typing (MLST), semi-automated repetitive sequence-based PCR (rep-PCR) (Diversilab), plasmid replicon typing, serotyping and screening for multiple resistance determinants. The isolates were selected to represent different strain clusters (defined by >80% similarity) according to pulsed-field gel electrophoresis. MLST identified three major clonal complexes (CCs); CC147 (n = 8), CC18 (n = 5) and CC14 (n = 3). Plasmid replicon typing showed that IncA/C and/or IncFIIK replicons were detected among isolates in each of the major CCs. Good concordance was observed between semi-automated-rep PCR genotyping and MLST.

KW - MLST

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KW - Metallo-β-lactamase

KW - AmpC

KW - Plasmid replicon

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