Dissemination of blaVIM in Greece at the peak of the epidemic of 2005-2006: Clonal expansion of Klebsiella pneumoniae clonal complex 147

C. M. Hasan, A. Turlej-Rogacka, A. C. Vatopoulos, P. Giakkoupi, M. Maatallah, C. G. Giske

Research output: Contribution to journalArticleOtherpeer-review

27 Citations (Scopus)

Abstract

VIM-producing Klebsiella pneumoniae (n = 21) isolated from ten Greek hospitals during 2003-2007 were characterized with multilocus sequence typing (MLST), semi-automated repetitive sequence-based PCR (rep-PCR) (Diversilab), plasmid replicon typing, serotyping and screening for multiple resistance determinants. The isolates were selected to represent different strain clusters (defined by >80% similarity) according to pulsed-field gel electrophoresis. MLST identified three major clonal complexes (CCs); CC147 (n = 8), CC18 (n = 5) and CC14 (n = 3). Plasmid replicon typing showed that IncA/C and/or IncFIIK replicons were detected among isolates in each of the major CCs. Good concordance was observed between semi-automated-rep PCR genotyping and MLST.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalClinical Microbiology and Infection
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 2014
Externally publishedYes

Keywords

  • MLST
  • Clonal complex
  • Metallo-β-lactamase
  • AmpC
  • Plasmid replicon

Cite this