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Dissecting the organization of a β-barrel assembly machinery (BAM) complex by neutron reflectometry

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Abstract

The β-barrel assembly machinery (BAM) complex in Gram-negative bacteria facilitates the assembly of β-barrel proteins into the outer membrane. Understanding the protein-protein interactions within this complex is essential for unravelling its functional mechanisms. Here, we present the use of neutron reflectometry for investigating the organization of β-barrel membrane protein complexes in the membrane environment. The spatial organization, protein positioning, protein-lipid interactions, and conformational changes within the complex can be elucidated by this method.

Original languageEnglish
Title of host publicationTransmembrane β-Barrel Proteins
Subtitle of host publicationMethods and Protocols
EditorsRaffaele Ieva
Place of PublicationNew York NY USA
PublisherSpringer Science + Business Media
Chapter17
Pages273-290
Number of pages18
Volume2778
ISBN (Electronic)9781071637340
ISBN (Print)9781071637333
DOIs
Publication statusPublished - 2024

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume2778
ISSN (Print)1064-3745

Keywords

  • BAM complex
  • Biomimetic membrane
  • Gram-negative bacteria
  • Neutron reflectometry
  • Outer membrane
  • Protein interactions
  • β-barrel assembly machinery

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