Projects per year
Abstract
Insecticide resistance is one of the most prevalent examples of anthropogenic genetic change, yet our understanding of metabolic-based resistance remains limited by the analytical challenges associated with rapidly tracking the in vivo metabolites of insecticides at nonlethal doses. Here, using twin ion mass spectrometry analysis of the extracts of whole Drosophila larvae and excreta, we show that (i) eight metabolites of the neonicotinoid insecticide, imidacloprid, can be detected when formed by susceptible larval genotypes and (ii) the specific overtranscription of a single gene product, Cyp6g1, associated with the metabolic resistance to neonicotinoids, results in a significant increase in the formation of three imidacloprid metabolites that are formed in C-H bond activation reactions; that is, Cyp6g1 is directly linked to the enhanced metabolism of imidacloprid in vivo. These results establish a rapid and sensitive method for dissecting the metabolic machinery of insects by directly linking single gene products to insecticide metabolism.
Original language | English |
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Pages (from-to) | 3525-3532 |
Number of pages | 8 |
Journal | Analytical Chemistry |
Volume | 86 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1 Apr 2014 |
Externally published | Yes |
Projects
- 1 Finished
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ARC Centre of Excellence - Centre for Free Radical Chemistry and Biotechnology
Scammells, P., Angus, J., Bottle, S., Coote, M. L., Davies, M. J., Easton, C. J., Hearn, M., Jackson, R., O'Hair, R. A. J., Radom, L., Schiesser, C., Sherburn, M. S., Wille, U., Barker, P., Jarrott, B., Rizzardo, E., Rogers, P. A. W. & Such, C. H.
Australian Research Council (ARC), Monash University
1/07/05 → 30/06/10
Project: Research