Disruption of Tsc2 in oocytes leads to overactivation of the entire pool of primordial follicles

Deepak Adhikari, Gilian Flohr, Nagaraju Gorre, Yan Shen, Hairu Yang, Eva Lundin, Zijian Lan, Michael J. Gambello, Kui Liu

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125 Citations (Scopus)

Abstract

To maintain the length of reproductive life in a woman, it is essential that most of her ovarian primordial follicles are maintained in a quiescent state to provide a continuous supply of oocytes. However, our understanding of the molecular mechanisms that control the quiescence and activation of primordial follicles is still in its infancy. In this study, we provide some genetic evidence to show that the tumor suppressor tuberous sclerosis complex 2 (Tsc2), which negatively regulates mammalian target of rapamycin complex 1 (mTORC1), functions in oocytes to maintain the dormancy of primordial follicles. In mutant mice lacking the Tsc2 gene in oocytes, the pool of primordial follicles is activated prematurely due to elevated mTORC1 activity in oocytes. This results in depletion of follicles in early adulthood, causing premature ovarian failure (POF). Our results suggest that the Tsc1-Tsc2 complex mediated suppression of mTORC1 activity is indispensable for maintenance of the dormancy of primordial follicles, thus preserving the follicular pool, and that mTORC1 activity in oocytes promotes follicular activation. Our results also indicate that deregulation of Tsc/mTOR signaling in oocytes may cause pathological conditions of the ovary such as infertility and POF.

Original languageEnglish
Article numbergap092
Pages (from-to)765-770
Number of pages6
JournalMolecular Human Reproduction
Volume15
Issue number12
DOIs
Publication statusPublished - 20 Oct 2009
Externally publishedYes

Keywords

  • Follicular activation
  • Oocytes
  • Premature ovarian failure
  • Tsc/mTOR signaling

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