Disposable Coverslip for Rapid Throughput Screening of Malaria Using Attenuated Total Reflection spectroscopy

Thulya Chakkumpulakkal Puthan Veettil, Kamila Kochan, Karen J. Edler, Paul De Bank, Philip Heraud, Bayden R. Wood

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Malaria is considered to be one of the most catastrophic health issues in the whole world. Vibrational spectroscopy is a rapid, robust, label-free, inexpensive, highly sensitive, nonperturbative, and nondestructive technique with high diagnostic potential for the early detection of disease agents. In particular, the fingerprinting capability of attenuated total reflection spectroscopy is promising as a point-of-care diagnostic tool in resource-limited areas. However, improvements are required to expedite the measurements of biofluids, including the drying procedure and subsequent cleaning of the internal reflection element to enable high throughput successive measurements. As an alternative, we propose using an inexpensive coverslip to reduce the sample preparation time by enabling multiple samples to be collectively dried together under the same temperature and conditions. In conjunction with partial least squares regression, attenuated total reflection spectroscopy was able to detect and quantify the parasitemia with root mean square error of cross-validation and R2 values of 0.177 and 0.985, respectively. Here, we characterize an inexpensive, disposable coverslip for the high throughput screening of malaria parasitic infections and thus demonstrate an alternative approach to direct deposition of the sample onto the internal reflection element.

Original languageEnglish
Pages (from-to)451-461
Number of pages11
JournalApplied Spectroscopy
Volume76
Issue number4
DOIs
Publication statusPublished - Apr 2022

Keywords

  • ATR FT-IR spectroscopy
  • attenuated total reflection Fourier transform infrared
  • Infrared substrates
  • malaria diagnosis
  • partial least squares regression
  • PLSR

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