Disperse-and-Collect Approach for the Type-Selective Detection of Matrix Metalloproteinases in Porous Silicon Resonant Microcavities

Fransiska S H Krismastuti, Melissa R. Dewi, Beatriz Prieto-Simon, Thomas Nann, Nicolas Hans Voelcker

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

We report on the design and testing of photonic biosensors for the type-selective detection of different types of matrix metalloproteinases (MMPs). The ability to detect a panel of different MMP types has important implications for prognosis of wound healing. We combine the immunocapture of MMPs on dispersed magnetic nanoparticles modified with antibodies specific for target MMPs (immuno-magNPs) with subsequent MMP detection upon fluorogenic peptide cleavage in porous silicon resonant microcavity (pSiRM) architectures. We report fast, sensitive, and type-selective detection of MMPs directly in wound fluid. This study sets the scene for downstream developments of multiparametric biosensors as point-of-care (POC) prognostic tools that may step-change chronic wound management.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalACS Sensors
Volume2
Issue number2
DOIs
Publication statusPublished - 24 Feb 2017
Externally publishedYes

Keywords

  • chronic wound management
  • magnetic nanoparticles
  • matrix metalloproteinases
  • multiparametric detection
  • porous silicon resonant microcavity

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