Discovery and Biosynthesis of Streptolateritic Acids A-D: Acyclic Pentacarboxylic Acids from Streptomyces sp. FXJ1.172 with Promising Activity against Potato Common Scab

Kairui Wang; Ning Liu; Minghao Liu; Pan Zhao; Naiqin Zhong; Gregory L. Challis; Ying Huang

doi:10.1021/acs.jafc.4c02572

Discovery and Biosynthesis of Streptolateritic Acids A-D: Acyclic Pentacarboxylic Acids from Streptomyces sp. FXJ1.172 with Promising Activity against Potato Common Scab

Kairui Wang
, Ning Liu
, Minghao Liu
, Pan Zhao
, Naiqin Zhong
, Gregory L. Challis
, Ying Huang

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

Potato common scab (PCS) is a widespread plant disease that lacks effective control measures. Using a small molecule elicitor, we activate the production of a novel class of polyketide antibiotics, streptolateritic acids A-D, in Streptomyces sp. FXJ1.172. These compounds show a promising control efficacy against PCS and an unusual acyclic pentacarboxylic acid structure. A gene cluster encoding a type I modular polyketide synthase is identified to be responsible for the biosynthesis of these metabolites. A cytochrome P450 (CYP) and an aldehyde dehydrogenase (ADH) encoded by two genes in the cluster are proposed to catalyze iterative oxidation of the starter-unit-derived methyl group and three of six branching methyl groups to carboxylic acids during chain assembly. Our findings highlight how activation of silent biosynthetic gene clusters can be employed to discover completely new natural product classes able to combat PCS and new types of modular polyketide synthase-based biosynthetic machinery.

Original language	English
Pages (from-to)	14760−14768
Number of pages	9
Journal	Journal of Agricultural and Food Chemistry
Volume	72
Issue number	26
DOIs	https://doi.org/10.1021/acs.jafc.4c02572
Publication status	Published - 3 Jul 2024

Keywords

aldehyde dehydrogenase
branching carboxyl groups
cytochrome P450
modular polyketide synthase
potato common scab
specialized metabolite
Streptomyces

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10.1021/acs.jafc.4c02572

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@article{887f47bf7ce3408688409dcaa4698830,

title = "Discovery and Biosynthesis of Streptolateritic Acids A-D: Acyclic Pentacarboxylic Acids from Streptomyces sp. FXJ1.172 with Promising Activity against Potato Common Scab",

abstract = "Potato common scab (PCS) is a widespread plant disease that lacks effective control measures. Using a small molecule elicitor, we activate the production of a novel class of polyketide antibiotics, streptolateritic acids A-D, in Streptomyces sp. FXJ1.172. These compounds show a promising control efficacy against PCS and an unusual acyclic pentacarboxylic acid structure. A gene cluster encoding a type I modular polyketide synthase is identified to be responsible for the biosynthesis of these metabolites. A cytochrome P450 (CYP) and an aldehyde dehydrogenase (ADH) encoded by two genes in the cluster are proposed to catalyze iterative oxidation of the starter-unit-derived methyl group and three of six branching methyl groups to carboxylic acids during chain assembly. Our findings highlight how activation of silent biosynthetic gene clusters can be employed to discover completely new natural product classes able to combat PCS and new types of modular polyketide synthase-based biosynthetic machinery.",

keywords = "aldehyde dehydrogenase, branching carboxyl groups, cytochrome P450, modular polyketide synthase, potato common scab, specialized metabolite, Streptomyces",

author = "Kairui Wang and Ning Liu and Minghao Liu and Pan Zhao and Naiqin Zhong and Challis, \{Gregory L.\} and Ying Huang",

note = "Funding Information: This work was supported by grants from the China Manned Space Engineering Program (CMSE) (YYWT-0801-EXP-08), the National Natural Science Foundation of China (32070001, 31700008, and 81773615), the International Co-Research Project of the Chinese Academy of Sciences (Grant no. 180GJHZ2023016MI), and a BBSRC China Partnering Award (BB/L010852/1). Publisher Copyright: {\textcopyright} 2024 American Chemical Society.",

year = "2024",

month = jul,

day = "3",

doi = "10.1021/acs.jafc.4c02572",

language = "English",

volume = "72",

pages = "14760−14768",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "26",

}

Discovery and Biosynthesis of Streptolateritic Acids A-D: Acyclic Pentacarboxylic Acids from Streptomyces sp. FXJ1.172 with Promising Activity against Potato Common Scab. / Wang, Kairui; Liu, Ning; Liu, Minghao et al.
In: Journal of Agricultural and Food Chemistry, Vol. 72, No. 26, 03.07.2024, p. 14760−14768.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Discovery and Biosynthesis of Streptolateritic Acids A-D

T2 - Acyclic Pentacarboxylic Acids from Streptomyces sp. FXJ1.172 with Promising Activity against Potato Common Scab

AU - Wang, Kairui

AU - Liu, Ning

AU - Liu, Minghao

AU - Zhao, Pan

AU - Zhong, Naiqin

AU - Challis, Gregory L.

AU - Huang, Ying

N1 - Funding Information: This work was supported by grants from the China Manned Space Engineering Program (CMSE) (YYWT-0801-EXP-08), the National Natural Science Foundation of China (32070001, 31700008, and 81773615), the International Co-Research Project of the Chinese Academy of Sciences (Grant no. 180GJHZ2023016MI), and a BBSRC China Partnering Award (BB/L010852/1). Publisher Copyright: © 2024 American Chemical Society.

PY - 2024/7/3

Y1 - 2024/7/3

N2 - Potato common scab (PCS) is a widespread plant disease that lacks effective control measures. Using a small molecule elicitor, we activate the production of a novel class of polyketide antibiotics, streptolateritic acids A-D, in Streptomyces sp. FXJ1.172. These compounds show a promising control efficacy against PCS and an unusual acyclic pentacarboxylic acid structure. A gene cluster encoding a type I modular polyketide synthase is identified to be responsible for the biosynthesis of these metabolites. A cytochrome P450 (CYP) and an aldehyde dehydrogenase (ADH) encoded by two genes in the cluster are proposed to catalyze iterative oxidation of the starter-unit-derived methyl group and three of six branching methyl groups to carboxylic acids during chain assembly. Our findings highlight how activation of silent biosynthetic gene clusters can be employed to discover completely new natural product classes able to combat PCS and new types of modular polyketide synthase-based biosynthetic machinery.

AB - Potato common scab (PCS) is a widespread plant disease that lacks effective control measures. Using a small molecule elicitor, we activate the production of a novel class of polyketide antibiotics, streptolateritic acids A-D, in Streptomyces sp. FXJ1.172. These compounds show a promising control efficacy against PCS and an unusual acyclic pentacarboxylic acid structure. A gene cluster encoding a type I modular polyketide synthase is identified to be responsible for the biosynthesis of these metabolites. A cytochrome P450 (CYP) and an aldehyde dehydrogenase (ADH) encoded by two genes in the cluster are proposed to catalyze iterative oxidation of the starter-unit-derived methyl group and three of six branching methyl groups to carboxylic acids during chain assembly. Our findings highlight how activation of silent biosynthetic gene clusters can be employed to discover completely new natural product classes able to combat PCS and new types of modular polyketide synthase-based biosynthetic machinery.

KW - aldehyde dehydrogenase

KW - branching carboxyl groups

KW - cytochrome P450

KW - modular polyketide synthase

KW - potato common scab

KW - specialized metabolite

KW - Streptomyces

UR - https://www.scopus.com/pages/publications/85196634466

U2 - 10.1021/acs.jafc.4c02572

DO - 10.1021/acs.jafc.4c02572

M3 - Article

C2 - 38899439

AN - SCOPUS:85196634466

SN - 0021-8561

VL - 72

SP - 14760−14768

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 26

ER -

Discovery and Biosynthesis of Streptolateritic Acids A-D: Acyclic Pentacarboxylic Acids from Streptomyces sp. FXJ1.172 with Promising Activity against Potato Common Scab

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Keywords

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ARC Centre of Excellence for Innovations in Peptide and Protein Science

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Discovery and Biosynthesis of Streptolateritic Acids A-D: Acyclic Pentacarboxylic Acids from Streptomyces sp. FXJ1.172 with Promising Activity against Potato Common Scab

Abstract

Keywords

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ARC Centre of Excellence for Innovations in Peptide and Protein Science

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