Direct plant gene delivery with a poly(amidoamine) dendrimer

Karthikeyan Pasupathy, Sijie Lin, Qian Hu, Hong Luo, Pu Chun Ke

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

Abstract

Plant gene delivery is challenging due to the presence of plant cell walls. Conventional means such as Agrobacterium infection, biolistic particle bombardment, electroporation, or polyethylene glycol attachment are often characterized by high cost, labor extensiveness, and a significant perturbation to the growth of cells. We have succeeded in delivering GFP-encoding plasmid DNA to turf-grass cells using poly(amidoamine) dendrimers. Our new scheme utilizes the physiochemical properties as well as the nanosize ofthe poly(amidoamine) dendrimer for direct and noninvasive gene delivery. The GFP gene was expressed in the plant cells as observed by confocal fluorescence microscopy. The transfection efficiency may be further improved by optimizing the pH of the cell culture medium and the molar ratio of the dendrimer to DNA. The use of the current delivery system can be extended to virtually all plant species having successful regeneration systems in place.

Original languageEnglish
Pages (from-to)1078-1082
Number of pages5
JournalBiotechnology Journal
Volume3
Issue number8
DOIs
Publication statusPublished - 1 Aug 2008
Externally publishedYes

Keywords

  • Dendrimer
  • Fluorescence
  • Gene delivery
  • Translocation

Cite this