Direct pituitary inhibition of prolactin secretion by dopamine and noradrenaline in sheep

G. B. Thomas, J. T. Cummins, B. W. Doughton, N. Griffin, G. A. Smythe, R. M. Gleeson, I. J. Clarke

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26 Citations (Scopus)

Abstract

The effects of dopamine, noradrenaline and 3,4-dihydroxyphenylacetic acid (DOPAC) on the release of prolactin were examined in ovariectomized ewes. Infusion of dopamine (0.5 or 1 μg/kg per min for 2 h i.v.) reduced plasma prolactin concentrations in a dose-dependent manner, whereas DOPAC (5 or 10 μg/kg per min for 2 h i.v.) had no effect. In a further series of experiments, ovariectomized hypothalamo-pituitary disconnected ewes were given dopamine or noradrenaline (each at 0.5 or 1 μg/kg per min for 2 h i.v.), and both amines reduced mean plasma concentrations of prolactin with similar potency in a dose-dependent manner. These effects were blocked by treatment with pimozide and prazosin respectively. During the infusion of dopamine, the peripheral plasma concentrations of DOPAC and 3,4-dihydroxyphenylethyleneglycol (DHPG) were increased (DOPAC, 22 ± 7 (S.E.M.) to 131 ± 11 nmol/l; DHPG, 2.9 ± 0.3 to 6.4 ± 0.2 nmol/l), but plasma concentrations of dopamine and noradrenaline did not change finally, administration of domperidone, a specific dopamine receptor antagonist that does not the blood-brain barrier, resulted in a sustained increase in plasma prolactin concentrations in ovariectomized ewes. We conclude that the secretion of prolactin from the pituitary gland is under dual inhibitory regulation by both dopamine and noradrenaline in the sheep.

Original languageEnglish
Pages (from-to)393-402
Number of pages10
JournalJournal of Endocrinology
Volume123
Issue number3
DOIs
Publication statusPublished - 1 Jan 1989
Externally publishedYes

Cite this

Thomas, G. B., Cummins, J. T., Doughton, B. W., Griffin, N., Smythe, G. A., Gleeson, R. M., & Clarke, I. J. (1989). Direct pituitary inhibition of prolactin secretion by dopamine and noradrenaline in sheep. Journal of Endocrinology, 123(3), 393-402. https://doi.org/10.1677/joe.0.1230393
Thomas, G. B. ; Cummins, J. T. ; Doughton, B. W. ; Griffin, N. ; Smythe, G. A. ; Gleeson, R. M. ; Clarke, I. J. / Direct pituitary inhibition of prolactin secretion by dopamine and noradrenaline in sheep. In: Journal of Endocrinology. 1989 ; Vol. 123, No. 3. pp. 393-402.
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Thomas, GB, Cummins, JT, Doughton, BW, Griffin, N, Smythe, GA, Gleeson, RM & Clarke, IJ 1989, 'Direct pituitary inhibition of prolactin secretion by dopamine and noradrenaline in sheep', Journal of Endocrinology, vol. 123, no. 3, pp. 393-402. https://doi.org/10.1677/joe.0.1230393

Direct pituitary inhibition of prolactin secretion by dopamine and noradrenaline in sheep. / Thomas, G. B.; Cummins, J. T.; Doughton, B. W.; Griffin, N.; Smythe, G. A.; Gleeson, R. M.; Clarke, I. J.

In: Journal of Endocrinology, Vol. 123, No. 3, 01.01.1989, p. 393-402.

Research output: Contribution to journalArticleResearchpeer-review

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Thomas GB, Cummins JT, Doughton BW, Griffin N, Smythe GA, Gleeson RM et al. Direct pituitary inhibition of prolactin secretion by dopamine and noradrenaline in sheep. Journal of Endocrinology. 1989 Jan 1;123(3):393-402. https://doi.org/10.1677/joe.0.1230393