Direct observation of nanoparticle-ubiquitin corona formation

Feng Ding, Slaven Radic, Ran Chen, Pengyu Chen, Nicholas K. Geitner, Jared M. Brow, Pu Chun Ke

Research output: Chapter in Book/Report/Conference proceedingConference PaperOtherpeer-review

Abstract

Upon entering physiological environments, nanoparticles readily assume the form of a nanoparticle-protein corona that dictates their biological identity. Understanding the structure and dynamics of nanoparticle-protein corona is essential for predicting the fate, transport, and toxicity of nanomaterials in living systems and for enabling the vast applications of nanomedicine. We combined multiscale molecular dynamics simulations and complementary experiments to characterize the silver nanoparticle-ubiquitin corona formation. Specifically, ubiquitins competed with citrates for the nanoparticle surface and bound to the particle in a specific manner. The specific binding between a silver nanoparticle and ubiquitin is governed by electrostatic interactions as observed in previous experiments. Under a high protein/nanoparticle stoichiometry, ubiquitins formed a multilayer corona on the particle surface. The binding exhibited a stretched-exponential behavior, suggesting a rich protein-nanoparticle binding kinetics. Furthermore, the binding destabilized the a-helices while increasing the β-sheets of the proteins. Our results revealed the structural and dynamic complexities of nanoparticle-protein corona formation and shed light on the origin of nanotoxicity.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages583-586
Number of pages4
Volume2
Publication statusPublished - 2013
Externally publishedYes
EventNanotechnology Conference and Trade Show (Nanotech) 2013 - Washington, United States of America
Duration: 12 May 201316 May 2013

Conference

ConferenceNanotechnology Conference and Trade Show (Nanotech) 2013
Abbreviated titleNanotech 2013 / NSTI 2013
CountryUnited States of America
CityWashington
Period12/05/1316/05/13
OtherNanotechnology 2013: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013

Keywords

  • Multiscale molecular dynamics
  • Nanoparticle-protein corona
  • Nanotechnology
  • Nanotoxicity
  • Protein conformational change

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