TY - JOUR
T1 - Differentiation of multipotent mesenchymal precursors and skeletal myoblasts from human embryonic stem cells.
AU - Stavropoulos, Maria Elena
AU - Mengarelli, Isabella
AU - Barberi, Tiziano
PY - 2009
Y1 - 2009
N2 - This unit describes a protocol for the derivation of multipotent mesenchymal precursors from human embryonic stem cells (hESCs). hESCs cultured at low density in the presence of a chemically defined serum-free medium are induced to adopt an endomesodermal fate and later a mesenchymal phenotype. FACS sorting for the surface antigen CD73 is used to purify mesenchymal precursors able to differentiate into fat, bone, cartilage, and skeletal muscle cells. Enrichment in mesenchymal precursors with a myogenic potential is achieved via an additional FACS sorting for the embryonic skeletal muscle surface marker N-CAM.
AB - This unit describes a protocol for the derivation of multipotent mesenchymal precursors from human embryonic stem cells (hESCs). hESCs cultured at low density in the presence of a chemically defined serum-free medium are induced to adopt an endomesodermal fate and later a mesenchymal phenotype. FACS sorting for the surface antigen CD73 is used to purify mesenchymal precursors able to differentiate into fat, bone, cartilage, and skeletal muscle cells. Enrichment in mesenchymal precursors with a myogenic potential is achieved via an additional FACS sorting for the embryonic skeletal muscle surface marker N-CAM.
UR - http://onlinelibrary.wiley.com/doi/10.1002/9780470151808.sc01f08s9/pdf
U2 - 10.1002/9780470151808.sc01f08s9
DO - 10.1002/9780470151808.sc01f08s9
M3 - Article
VL - Chapter 1
SP - 1
EP - 10
JO - Current Protocols in Stem Cell Biology
JF - Current Protocols in Stem Cell Biology
SN - 1941-7322
IS - Unit 1F.8
ER -